比较转录组分析揭示了柑橘木虱（Diaphorina citri）在热激下差异表达的基因。

Comparative transcriptome analysis reveals differentially expressed genes in the Asian citrus psyllid (Diaphorina citri) upon heat shock.

机构信息

Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing 400716, China; International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.

Guangxi Key Laboratory of Citrus Biology, Guangxi Citrus Research Institute, Gulin, Guangxi 541004, China.

出版信息

Comp Biochem Physiol Part D Genomics Proteomics. 2019 Jun;30:256-261. doi: 10.1016/j.cbd.2019.03.009. Epub 2019 Apr 1.

DOI:10.1016/j.cbd.2019.03.009

PMID:30965232

Abstract

Temperature is an important factor influencing insect distribution. In the tropical and subtropical regions, insects always suffer the extreme temperature. Therefore, appropriate molecular response to temperature change is crucial for their survival. To understand how Diaphorina citri responds to high temperature at the molecular level, we conducted a comparative analysis of the transcriptomes of D. citri under room temperature and 40 °C heat shock treatment. The RNA sequencing analysis identified a total of 451 differentially expressed genes upon heat stress, including 167 down-regulated genes and 284 up-regulated genes. Thermal stress mainly significantly induced the transcription of molecular chaperone, protein biosynthesis and oxidation resistance, including members of the heat shock protein families, ATPases, and detoxifying enzymes. This study provides a preliminary insight into the transcriptional response of D. citri to heat stress and provides a foundation for the future functional validation of genes involved in thermotolerance in this important insect pest in tropical and subtropical regions.

摘要

温度是影响昆虫分布的一个重要因素。在热带和亚热带地区，昆虫经常遭受极端温度。因此，对温度变化的适当分子响应对它们的生存至关重要。为了了解柑橘木虱如何在分子水平上对高温做出响应，我们对柑橘木虱在室温（25℃）和 40℃热激处理下的转录组进行了比较分析。RNA 测序分析共鉴定到 451 个差异表达基因，其中包括 167 个下调基因和 284 个上调基因。热应激主要显著诱导分子伴侣、蛋白质生物合成和抗氧化的转录，包括热休克蛋白家族、ATP 酶和解毒酶的成员。本研究初步揭示了柑橘木虱对热应激的转录响应，并为今后验证热带和亚热带地区这一重要害虫中与耐热性相关的基因的功能提供了基础。