College of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, China; Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; National Research and Development Center for Edible Fungi Processing (Wuhan), Wuhan 430064, China.
Int J Biol Macromol. 2020 Aug 1;156:1425-1434. doi: 10.1016/j.ijbiomac.2019.11.183. Epub 2019 Nov 21.
Water-soluble polysaccharides from Schizophyllum commune (SPGs) were extracted using different methods including hot-water extraction (HWE), ultrasound-assisted extraction (UE), microwave-assisted extraction (ME) and high pressure extraction (HPE). The physicochemical characteristics, antioxidant activity and hypoglycemic activity of four different SPGs were investigated. The results indicated that the polysaccharides yield (8.26%), uronic acid content (3.0%), total polysaccharides content (67.96%) and molecular weight (52.7 kDa) of SPGs extracted by HWE were higher than those obtained by other methods. FT-IR spectra and conformation information of four SPGs were similar, however, the color characteristics, the monosaccharide species and molar ratios, the solubility and micro-structure of each SPGs were significantly different. Though the molecular weight (11.0 kDa) of UE-SPGs was lowest, it showed the highest antioxidant activity and hypoglycemic activity. It could be considered that the UE extraction method might be a good potential extraction technique for the SPGs with high bioactivities.
从裂褶菌（Schizophyllum commune）中提取的水溶性多糖（SPGs）采用了不同的方法，包括热水提取（HWE）、超声辅助提取（UE）、微波辅助提取（ME）和高压提取（HPE）。研究了四种不同 SPG 的理化特性、抗氧化活性和降血糖活性。结果表明，HWE 提取的多糖得率（8.26%）、糖醛酸含量（3.0%）、总多糖含量（67.96%）和分子量（52.7 kDa）均高于其他方法。四种 SPG 的 FT-IR 光谱和构象信息相似，但每种 SPG 的颜色特征、单糖种类和摩尔比、溶解度和微观结构差异显著。尽管 UE-SPGs 的分子量（11.0 kDa）最低，但它表现出最高的抗氧化活性和降血糖活性。因此，可以认为 UE 提取方法可能是一种具有高生物活性的 SPG 的潜在良好提取技术。
