Nkenfou Celine Nguefeu, Abange William Baiye, Gonsu Hortense Kamga, Kamgaing Nelly, Lyonga Emilia Mbamyah, Anoubissi Jean de Dieu, Ndjolo Alexis, Koki Paul
Chantal Biya's International Reference Centre (CBIRC), Centre for HIV/AIDS Prevention and Management, Yaounde, Cameroon.
Department of Biological Sciences, Higher Teachers Training College, University of Yaounde I, Cameroon.
Pan Afr Med J. 2019 Sep 11;34:24. doi: 10.11604/pamj.2019.34.24.15039. eCollection 2019.
HIV infection is characterized by changes in the composition and functions of gut microbiota. We carried out a study aiming at comparing the compositional changes of the digestive flora of HIV infected infants versus that of non infected infants in Cameroon.
A case-control study was carried out during which stool sample was collected from each participant after obtaining the proxy consent. Stools were cultured using aerobic, strict anaerobic, 10% CO2 and micro-aerophilic conditions and specific culture media and bacteria were identified biochemically. Fisher's exact test was used for data analyses.
From the 80 infants enrolled for the study, 33 (41.3%) were HIV positive. A statistically significant difference was observed between the number of infected versus non infected infants harboring the following bacteria: spp. (P=0.009); spp. (p<0.001); (p<0.001); (<0.001); (p=0.006) and spp. (P=0.015). Among infected infants, WHO-stage 3 and 4 infants harbored more opportunistic bacteria than stage 1 and stage 2 and spp. population was depleted as the disease progresses, although not significantly. There was an imbalance in bacteria flora in HIV infected infants harboring qualitatively more bacteria including more opportunistic and pathogenic bacteria than in HIV non-infected infants.
HIV infected infants presented a qualitatively different flora from HIV non infected infants. They habored more pathogenic bacteria Than non infected infants. Systematic stool culture could benefit for follow-up of HIV infected infants to reduce the risk of gastrointestinal disorders and thus the risk of high morbidity or high mortability.
HIV感染的特征是肠道微生物群的组成和功能发生变化。我们开展了一项研究，旨在比较喀麦隆HIV感染婴儿与未感染婴儿消化菌群的组成变化。
进行了一项病例对照研究，在获得代理同意后，从每位参与者收集粪便样本。粪便在需氧、严格厌氧、10%二氧化碳和微需氧条件下培养，并使用特定培养基培养，通过生化方法鉴定细菌。采用Fisher精确检验进行数据分析。
在纳入该研究的80名婴儿中，33名（41.3%）为HIV阳性。在携带以下细菌的感染婴儿与未感染婴儿数量之间观察到统计学上的显著差异： spp.（P = 0.009）； spp.（p < 0.001）； （p < 0.001）； （< 0.001）； （p = 0.006）和 spp.（P = 0.015）。在感染婴儿中，世界卫生组织3期和4期婴儿比1期和2期婴儿携带更多的机会性细菌，并且随着疾病进展 spp.数量减少，尽管不显著。与未感染HIV的婴儿相比，感染HIV的婴儿菌群存在失衡，定性地含有更多细菌，包括更多的机会性和致病性细菌。
HIV感染婴儿的菌群在性质上与未感染HIV的婴儿不同。他们比未感染婴儿携带更多的病原菌。系统性粪便培养可能有益于HIV感染婴儿的随访，以降低胃肠道疾病风险，从而降低高发病率或高死亡率的风险。
