Darwesh Osama M, El-Hawary Ahmad S, El Kelany Usama S, El-Sherbiny Gamal M
Agricultural Microbiology Department, National Research Centre, Dokki, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Nasr city, Cairo, Egypt.
Biotechnol Rep (Amst). 2019 Oct 25;24:e00386. doi: 10.1016/j.btre.2019.e00386. eCollection 2019 Dec.
Application of thermostable alkaline protease to control the harmful nematodes was investigated in the current study. A total of 14 proteolytic actinomycetes were isolated from Egyptian harsh environments. Out of them, isolate G550 exhibited the highest proteolytic activity (528.9 U/ml). Protease from isolate G550 exhibited high nematicidal activity against under laboratory conditions and caused hydrolysis of JS cuticle. This isolate was identified using molecular techniques and deposited in GenBank under name of strain Hw G550 with accession number: MF152631. The G550 protease was extracted, characterized and applied under greenhouse conditions as nematicidal agent. This enzyme exhibited maximum activity and stability at alkaline pH (8) and thermal conditions (50-60 °C). Also, the results showed that, all treatments using protease caused a significant decrease in nematode reproduction and increasing in the plant properties. Finally, the thermo alkaliphilic protease could be used as bio-control agent against RKN.
本研究对热稳定碱性蛋白酶在控制有害线虫方面的应用进行了调查。从埃及恶劣环境中总共分离出14株蛋白水解放线菌。其中，分离株G550表现出最高的蛋白水解活性（528.9 U/ml）。来自分离株G550的蛋白酶在实验室条件下对[此处原文缺失对象]表现出高杀线虫活性，并导致JS角质层水解。该分离株通过分子技术进行鉴定，并以菌株Hw G550的名称保藏于GenBank，登录号为：MF152631。提取、表征了G550蛋白酶，并在温室条件下作为杀线虫剂应用。该酶在碱性pH值（8）和热条件（50 - 60°C）下表现出最大活性和稳定性。此外，结果表明，所有使用蛋白酶的处理均导致线虫繁殖显著减少，植物特性增强。最后，嗜热嗜碱蛋白酶可作为防治根结线虫的生物防治剂。
