Pollack M S, Hayes A, Mooney S, Pedersen N C, Cook R G
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.
Vet Immunol Immunopathol. 1988 Jul;19(1):79-91. doi: 10.1016/0165-2427(88)90048-7.
The presence on feline cells of class I and class II I-E type major histocompatibility complex (MHC) homologues was demonstrated using cross-reacting monoclonal antibodies (mAb). The feline class I antigen homologues were detected with both immunofluorescent and biochemical techniques, using the anti-human class I mAb W6/32. The class I antigens were detected on in vitro cultured feline fibroblasts and lymphoid cells, but not on fresh lymphoid cells, apparently as a result of the association of bovine beta-2 microglobulin with feline class I heavy chains which generated the determinant(s) recognized by mAb W6/32. Class II I-E-like molecules could be detected with immunofluorescent techniques using the species cross-reactive anti-mouse I-E antibody 40D only when peripheral blood mononuclear cells were activated, for example, with the mitogens staphylococcus enterotoxin A or lipopolysaccharide. The predominant expression of I-A-like molecules by resting class II-positive feline cells could explain some of the functional difference we have seen in comparison with those of most other mammalian species.
利用交叉反应单克隆抗体（mAb）证实了猫细胞中存在I类和II类I-E型主要组织相容性复合体（MHC）同源物。使用抗人I类mAb W6/32，通过免疫荧光和生化技术检测到了猫I类抗原同源物。在体外培养的猫成纤维细胞和淋巴细胞上检测到了I类抗原，但在新鲜淋巴细胞上未检测到，这显然是由于牛β-2微球蛋白与猫I类重链结合，产生了mAb W6/32识别的决定簇。只有当外周血单个核细胞被激活时，例如用促细胞分裂剂肠毒素A或脂多糖激活，才能使用种间交叉反应抗小鼠I-E抗体40D通过免疫荧光技术检测到II类I-E样分子。静止的II类阳性猫细胞主要表达I-A样分子，这可以解释我们所观察到的与大多数其他哺乳动物物种相比的一些功能差异。
