College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350019, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan' Crops/Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice in South China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Fuzhou Branch, National Center of Rice Improvement of China/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, 350003, China.
Biochem Biophys Res Commun. 2020 Feb 5;522(2):448-455. doi: 10.1016/j.bbrc.2019.11.125. Epub 2019 Nov 23.
Trichomes are specialized epidermal cells that play crucial roles in resisting environmental stress and enhancing plant development. In Arabidopsis thaliana, the main genes controlling trichome formation have been consecutively identified. However, few genes like this were reported in rice. In this study, we identified the hairy phenotype of indica variety 75-1-127. This was used to construct a segregation population with a cross of hairless variety Minghui63 (MH63) to fine map the trichome formation genes. Genetic analysis indicated that hairy phenotype was controlled by a pair of dominant genes on chromosome 6, which was designated as GLABRA6 (OsGL6). OsGL6 was an allele of HL6 gene whose sequences containing rich variations in genomes. Compared to wild type, the overexpressing transgenic lines revealed that OsGL6 promoted trichome initiation. We found that OsGL6 interacted with serine/threonine protein kinase OSK3 (OSK3) or COP9 signalosome complex subunit 5a (CSN5) in yeast. These results provide potential information for understanding the molecular mechanism of trichome formation in rice.
表皮毛是一种特化的表皮细胞，在抵抗环境胁迫和促进植物发育方面起着关键作用。在拟南芥中，控制表皮毛形成的主要基因已被相继鉴定。然而，在水稻中，像这样的基因报道较少。在这项研究中，我们鉴定了籼稻品种 75-1-127 的多毛表型。利用这个品种与无毛品种明恢 63（MH63）杂交构建分离群体，对表皮毛形成基因进行精细定位。遗传分析表明，多毛表型由一对位于第 6 号染色体上的显性基因控制，该基因被命名为 GLABRA6（OsGL6）。OsGL6 是 HL6 基因的一个等位基因，其基因组中含有丰富的变异序列。与野生型相比，过表达转基因系显示 OsGL6 促进了表皮毛的起始。我们发现 OsGL6 在酵母中与丝氨酸/苏氨酸蛋白激酶 OSK3（OSK3）或 COP9 信号小体复合物亚基 5a（CSN5）相互作用。这些结果为理解水稻表皮毛形成的分子机制提供了潜在信息。
