Park Jae-Ryoung, Seo Jeonghwan, Lee Chang-Min, Ji Hyeonso, Jeong O-Young, Park Hyun-Su
Crop Breeding Division, Department of Food Crop Science, National Institute of Food & Crop Science, Rural Development Administration, Wanju, 55365, Republic of Korea.
Digital Breeding Convergence Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, Republic of Korea.
BMC Plant Biol. 2025 Aug 2;25(1):1017. doi: 10.1186/s12870-025-07113-5.
Rice is the primary source of calories for a large portion of the global population. Most rice hulls are of the trichome-type and produce dust, which can cause respiratory allergies and environmental concerns during handling. By contrast, glabrous-type hulls reduce storage volume and minimise dust, making processing cleaner and more eco-friendly. Despite the advantages of glabrous rice as a breeding resource, no molecular markers are available for the effective selection of this trait.
In this study, we developed a novel cleaved amplified polymorphic sequence (CAPS) marker specifically designed to select glabrous hulls in rice. Using the sequence information of the Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) marker KJ05_001, closely linked to GLR1, we identified an MseI recognition site for targeted digestion. This sequence was used to create the GLR1_CAPS marker, showing tight linkage to GLR1. The effectiveness of this marker was tested in 290 diverse rice germplasm lines, confirming its broad applicability.
Overall, the GLR1_CAPS marker is an efficient and reliable tool for breeding programs focused on the development of glabrous rice varieties. By facilitating accurate selection of this trait, the developed marker offers substantial improvements in rice storage, handling, and processing, contributing to more sustainable and allergy-friendly agricultural practices. This novel molecular marker represents an important advancement in rice breeding and opens new avenues for the development of rice varieties with reduced environmental and health impacts.
大米是全球很大一部分人口的主要热量来源。大多数稻壳是具毛状体类型的，会产生粉尘，在处理过程中会引发呼吸道过敏和环境问题。相比之下，无毛类型的稻壳减少了储存体积并将粉尘降至最低，使加工更清洁、更环保。尽管无毛稻作为一种育种资源具有诸多优势，但尚无用于有效选择该性状的分子标记。
在本研究中，我们开发了一种新型的酶切扩增多态性序列（CAPS）标记，专门用于在水稻中选择无毛稻壳。利用与GLR1紧密连锁的竞争性等位基因特异性聚合酶链反应（KASP）标记KJ05_001的序列信息，我们确定了一个用于靶向消化的MseI识别位点。该序列用于创建GLR1_CAPS标记，显示出与GLR1紧密连锁。在290个不同的水稻种质系中测试了该标记的有效性，证实了其广泛的适用性。
总体而言，GLR1_CAPS标记是专注于无毛水稻品种培育的育种计划的一种高效且可靠的工具。通过促进对该性状的准确选择，所开发的标记在水稻储存、处理和加工方面有显著改进，有助于实现更可持续且对过敏友好的农业实践。这种新型分子标记代表了水稻育种的一项重要进展，并为开发对环境和健康影响较小的水稻品种开辟了新途径。
