SAM 与 基因 5'UTR mRNA 之间的相互作用调节 的翻译。
Interactions between SAM and the 5' UTR mRNA of the gene regulate translation in .
机构信息
Fudan University Pudong Medical Center, Pudong and Key Laboratory of Medical Epigenetics and Metabolism, Institute of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
出版信息
RNA. 2020 Feb;26(2):150-161. doi: 10.1261/rna.072983.119. Epub 2019 Nov 25.
The 5' untranslated region (5' UTR) of eukaryotic mRNA plays an important role in translation. Here we report the function of the 5' UTR mRNA of -adenosylmethionine synthetase () in translational modulation in the presence of SAM in fission yeast Reporter assays, binding and chemical probing experiments, and mutational analysis show that the 5' UTR mRNA of binds to SAM to effect translation. Translational modulation is dependent on a tertiary structure transition in the RNA upon SAM binding. The characterization of such an RNA that is directly associated with an essential metabolic process in eukaryotes provides additional evidence that ligand binding by RNAs plays an important role in eukaryotic gene regulation.
真核生物 mRNA 的 5'非翻译区 (5'UTR) 在翻译中起着重要作用。在这里，我们报告了在裂殖酵母中 SAM 存在的情况下，-腺苷甲硫氨酸合成酶 () 的 5'UTR mRNA 在翻译调节中的作用。报告基因测定、结合和化学探测实验以及突变分析表明，SAM 结合到 5'UTR mRNA 上，从而影响翻译。翻译调节依赖于 SAM 结合时 RNA 中的三级结构转变。这种 RNA 的特征是与真核生物中必需的代谢过程直接相关，这为配体结合 RNA 在真核生物基因调控中发挥重要作用提供了额外的证据。
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