Biondo-Simões Maria de Lourdes Pessole, Henning Júnior Lucélio, Boen Bruno Russiano de Oliveira, Prado Julyana Leoni do, Costa Luiza Rodrigues da, Robes Rogério Ribeiro, Ioshii Sérgio Ossamu
Universidade Federal do Paraná (UFPR), Centro de Ciências da Saúde, Curso de Medicina, Departamento de Cirurgia, Curitiba, PR, Brasil.
Universidade Federal do Paraná (UFPR), Centro de Ciências da Saúde, Curso de Medicina, Curitiba, PR, Brasil.
Rev Col Bras Cir. 2019 Nov 25;46(5):e20192245. doi: 10.1590/0100-6991e-20192245. eCollection 2019.
to compare the healing by second intention under the effects of topical application of honey, copaíba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) with a control group in rats.
we carried out a skin resection, 1cm in diameter, on the back of 40 rats allocated to four groups of ten animals. All wounds were cleaned daily with 2ml of 0.9% NaCl solution. The first group (control - GC) was restricted to such procedure. In the wounds of the second (GM), third (GO) and fourth groups (GF), after cleaning, we respectively applied 1ml of honey, 1ml of copaíba oil-resin and 1ml of cream containing fibrinolysin, deoxyribonuclease and chloramphenicol. The wounds were occluded with sterile gauze. Immediately after the incision and on days three, seven and 14 of the experiment, the wounds were copied and contraction was analyzed using planimetry. After euthanasia, we histologically evaluated the inflammatory reaction and collagen in the scars.
the reduction of the wound area of GM (p=0.003), GO (p=0.011) and GF (p=0.002) were higher than the GC. The amount of type-I collagen present in GM and GO was higher than in GC and GF groups (p<0.05). There was a predominance of chronic inflammatory stage in GM (p=0.004), GO (p<0.001) and GF (p=0.003) when compared with GC.
the topical use of honey and copaíba oil-resin increases wound contraction, the presence of type-I collagen and accelerates healing.
比较在大鼠中，局部应用蜂蜜、古巴香脂油树脂和一种商业产品（纤维蛋白溶酶、脱氧核糖核酸酶和氯霉素）与对照组相比，二期愈合的情况。
我们在40只大鼠的背部进行了直径为1cm的皮肤切除术，将其分为四组，每组10只动物。所有伤口每天用2ml 0.9%的氯化钠溶液清洗。第一组（对照组-GC）仅进行该清洗操作。在第二组（GM）、第三组（GO）和第四组（GF）的伤口清洗后，我们分别应用1ml蜂蜜、1ml古巴香脂油树脂和1ml含有纤维蛋白溶酶、脱氧核糖核酸酶和氯霉素的乳膏。伤口用无菌纱布覆盖。在切口后立即以及实验的第3、7和14天，对伤口进行拍照，并使用面积测量法分析收缩情况。安乐死后，我们对瘢痕中的炎症反应和胶原蛋白进行组织学评估。
GM组（p=0.003）、GO组（p=0.011）和GF组（p=0.002）的伤口面积减少幅度高于GC组。GM组和GO组中I型胶原蛋白的含量高于GC组和GF组（p<0.05）。与GC组相比，GM组（p=0.004）、GO组（p<0.001）和GF组（p=0.003）主要处于慢性炎症阶段。
局部使用蜂蜜和古巴香脂油树脂可增加伤口收缩、I型胶原蛋白的含量并加速愈合。
