Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Biomed Res Int. 2019 Nov 5;2019:2365416. doi: 10.1155/2019/2365416. eCollection 2019.
Cartilage dysplasia is one of the important reasons for the weakness of pig limbs and hooves. Porcine rickets with weak limbs and hooves bring huge economic losses to the pig industry. However, research on the development of pig cartilage is lacking. This study investigated the key genes and molecular mechanisms involved in cartilage development via an RNA-seq technique. Samples of proximal tibia cartilage were collected from three normal piglets with 1 day, 14 days, and 28 days of age, respectively, and then these samples were divided into two comparison groups (1-day vs. 14-day group, 14-day vs. 28-day group). Through the transcriptome analysis, 108 differentially expressed genes (DEGs), such as , were obtained from 1-day vs. 14-day comparison group, and 3602 DEGs were obtained from 14-day vs. 28-day comparison group, including , , and . The gene ontology (GO) functional and KEGG pathway enrichment revealed that many functions of DEGs were related to bone development. The pathways of DEGs from Day 1 vs. Day 14 were mainly enriched in mineral absorption, but the DEGs of Day 14 vs. Day 28 were enriched in osteoclast differentiation. Then, the expression patterns of six candidate genes were verified via qPCR. In conclusion, candidate genes affecting cartilage development in Yorkshire pigs were obtained by transcriptome analysis, and the clues showed that Day 14 to Day 28 is a more active and extensive period in cartilage developments, which played a key role in revealing the molecular mechanism of pig cartilage development basis, also compensating for vacancies in cartilage research.
软骨发育不良是猪四肢和蹄子虚弱的重要原因之一。患有佝偻病、四肢和蹄子虚弱的猪给养猪业带来了巨大的经济损失。然而，关于猪软骨发育的研究还很缺乏。本研究通过 RNA-seq 技术研究了软骨发育过程中的关键基因和分子机制。分别从 3 头正常仔猪的近胫骨软骨中采集 1 日龄、14 日龄和 28 日龄的样本，然后将这些样本分为两组进行比较（1 日龄与 14 日龄组、14 日龄与 28 日龄组）。通过转录组分析，从 1 日龄与 14 日龄比较组中获得了 108 个差异表达基因（DEGs），如 ，而从 14 日龄与 28 日龄比较组中获得了 3602 个 DEGs，包括 ， ，和 。基因本体（GO）功能和 KEGG 通路富集分析表明，DEGs 的许多功能与骨骼发育有关。DEGs 从第 1 天到第 14 天的通路主要富集在矿物质吸收中，而第 14 天到第 28 天的 DEGs 则富集在破骨细胞分化中。然后，通过 qPCR 验证了六个候选基因的表达模式。综上所述，通过转录组分析获得了影响约克夏猪软骨发育的候选基因，这些线索表明第 14 天到第 28 天是软骨发育更为活跃和广泛的时期，这对揭示猪软骨发育的分子机制基础起到了关键作用，也弥补了软骨研究的空白。
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