206, Structural Biology Laboratory, Centre for Biomedical Research, School of Biosciences & Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India.
Geroscience. 2020 Feb;42(1):271-285. doi: 10.1007/s11357-019-00135-6. Epub 2019 Nov 30.
Wild-type Canton-S flies of Drosophila melanogaster were treated with ellagic acid at 100 μM and 200 μM concentrations. Longevity assay showed male flies fed with 200 μM ellagic acid displayed longer mean lifespan and maximum lifespan than control flies. Female flies fed with 200 μM ellagic acid laid less number of eggs than control. The eclosion time was less in female flies fed with 200 μM ellagic acid. Ellagic acid fed female flies performed better than male flies and control flies for heat shock tolerance and starvation stress. Male flies treated with 100 μM ellagic acid recovered faster from cold shock compared with control flies. Male and female flies treated with ellagic acid displayed increased survival following exposure to 5% hydrogen peroxide. Gene expression studies displayed upregulated expressions of CAT, dFOXO, ATG1, and SOD2 in ellagic acid-treated male flies, and upregulated expressions of dFOXO, CAT, and SOD2 in ellagic acid-treated female flies. Results from these studies show the pro-longevity effect of ellagic acid on Drosophila melanogaster.
野生型黑腹果蝇 Canton-S 品系在 100μM 和 200μM 浓度的鞣花酸处理下,寿命测定表明,喂食 200μM 鞣花酸的雄性果蝇比对照果蝇表现出更长的平均寿命和最大寿命。喂食 200μM 鞣花酸的雌性果蝇产卵数比对照少。喂食 200μM 鞣花酸的雌性果蝇的出蛹时间更短。在热休克耐受和饥饿应激方面,雌性果蝇的表现优于雄性果蝇和对照果蝇。与对照果蝇相比,100μM 鞣花酸处理的雄性果蝇从冷休克中恢复得更快。暴露于 5%过氧化氢后,雄性和雌性果蝇的存活率均有所提高。基因表达研究显示,鞣花酸处理的雄性果蝇中 CAT、dFOXO、ATG1 和 SOD2 的表达上调,鞣花酸处理的雌性果蝇中 dFOXO、CAT 和 SOD2 的表达上调。这些研究结果表明鞣花酸对黑腹果蝇具有延长寿命的作用。