Biology Program, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000, Thailand.
Thai Traditional Medicine Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000, Thailand.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Jun;244:108994. doi: 10.1016/j.cbpc.2021.108994. Epub 2021 Feb 4.
Aging is a significant risk factor that links to the genesis of human diseases. The capacity to scavenge free radicals and adapt to various stresses is essential for expanding living organisms' lifespan. The evidences on the promotion of longevity by dietary supplementation are growing. Drosophila or fruit fly is one of the most effective models for the evaluation of anti-aging compounds. Xanthohumol (XN) is a potential bioactive substance for the prevention and treatment of many diseases. The previous studies have reported its potent activities as antioxidant, anticancer, anti-inflammatory, antiviral, antibacterial antiplasmodial, and antiobesity. In this study, the effect of XN supplementation on the lifespan extension was investigated in Drosophila melanogaster. The effects of XN on the improvement of the recovery from cold and heat shock, the resistance to starvation stress, and free radical-induced oxidative stress in XN-treated flies were also evaluated. Results showed that supplementation with XN at 0.5 mg/mL diet extended the mean lifespan by 14.89%. This was consistent with a significant improvement of locomotor activity of the Drosophila fed with an XN-mixed diet compared with those fed with a control diet. XN supplementation significantly increased the antioxidant enzyme activities at both 25 and 40 days. Drosophila treated with XN exhibited increased survival after exposure to hydrogen peroxide and paraquat. Finally, XN supplementation improved the recovery from cold and heat shock, the resistance to starvation stress, and acetic acid-induced stress. The present study shows that dietary supplementation with XN revealed the longevity effect and ameliorated stress-induced mortality in Drosophila.
衰老是导致人类疾病发生的重要风险因素。清除自由基和适应各种应激的能力对于延长生物体的寿命至关重要。越来越多的证据表明，饮食补充剂可以促进长寿。果蝇是评估抗衰老化合物的最有效模型之一。黄烷酮（XN）是一种有潜力的预防和治疗多种疾病的生物活性物质。先前的研究报道了其作为抗氧化剂、抗癌、抗炎、抗病毒、抗细菌、抗疟原虫和抗肥胖的有效活性。在这项研究中，研究了 XN 补充剂对黑腹果蝇寿命延长的影响。还评估了 XN 对改善果蝇从冷、热休克中恢复、抵抗饥饿应激和自由基诱导的氧化应激的影响。结果表明，XN 以 0.5mg/ml 饮食补充剂可使平均寿命延长 14.89%。这与 XN 混合饮食喂养的果蝇的运动活性明显优于对照饮食喂养的果蝇一致。XN 补充剂在 25 天和 40 天均显著增加了抗氧化酶的活性。用 XN 处理的果蝇在暴露于过氧化氢和百草枯后存活率增加。最后，XN 补充剂改善了冷、热休克的恢复，抵抗饥饿应激和乙酸诱导的应激。本研究表明，XN 的饮食补充显示了其延长寿命的效果，并改善了果蝇应激诱导的死亡率。
