Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
PLoS One. 2019 Dec 2;14(12):e0225833. doi: 10.1371/journal.pone.0225833. eCollection 2019.
The understanding of factors affecting the gut bacterial communities in malaria vectors is essential for the design of vector control interventions, such as those based on a paratransgenic approach. One of the requirements of this method is the availability of bacteria from the mosquito susceptible to culture. Thus, the aim of this study was to evaluate the composition and structure of the culturable gut bacterial communities in field mosquitoes Anopheles albimanus from Colombia, in addition to generate a bacterial collection to further analyze microbial functional activity. Gut bacteria were isolated from An. albimanus larvae and adult mosquitoes collected in localities of the Atlantic and Pacific Coasts. The bacterial isolates were grouped in 28 morphospecies that corresponded to three phyla, three classes, nine families and 14 genera. The larvae guts from San Antero (Atlantic Coast) and Buenaventura (Pacific Coast) shared the genera Bacillus and Lysinibacillus and in adults, Bacillus and Bacillus cereus Group were registered in both localities. Gut bacterial richness was higher in adults from the Pacific with respect to the Atlantic Coast, while larval richness was similar in samples of both coasts. The Shannon index indicated uniformity in morphospecies abundances in both localities. Finally, the characterization of morphospecies from the gut of Anopheles albimanus mosquitoes from Colombia by culture-dependent methods complemented with 16S rRNA gene sequencing allowed the definition, at a finer resolution, of the composition and structure of these microbial communities. In addition, the obtained bacterial culture collection will allow further evaluation of the microorganisms for their potential as biocontrol agents.
了解影响疟疾传播媒介肠道细菌群落的因素对于设计媒介控制干预措施至关重要，例如基于转基因方法的干预措施。这种方法的一个要求是需要有易于培养的蚊子细菌。因此，本研究的目的是评估哥伦比亚野外白纹伊蚊肠道可培养细菌群落的组成和结构，此外还生成了细菌培养物集以进一步分析微生物功能活性。从在大西洋和太平洋沿岸收集的白纹伊蚊幼虫和成年蚊子中分离肠道细菌。将细菌分离株分为 28 个形态种，对应于三个门、三个纲、九个科和 14 个属。来自 San Antero（大西洋海岸）和 Buenaventura（太平洋海岸）的幼虫肠道共享芽孢杆菌属和短小芽孢杆菌属，而在成年蚊子中，在两个地方都记录了芽孢杆菌属和蜡状芽孢杆菌群。太平洋沿岸成年蚊子的肠道细菌丰富度高于大西洋海岸，而两个海岸的幼虫丰富度相似。Shannon 指数表明两个地方的形态种丰度均匀。最后，通过培养依赖方法和 16S rRNA 基因测序对哥伦比亚白纹伊蚊肠道的形态种进行了表征，从而更精细地定义了这些微生物群落的组成和结构。此外，获得的细菌培养物集将允许进一步评估这些微生物作为生物防治剂的潜力。
