伊朗璃眼蜱和钝缘蜱中柯克斯体（γ-变形菌：柯克斯体科）的分子检测。
Molecular detection of Coxiella (Gammaproteobacteria: Coxiellaceae) in Argas persicus and Alveonasus canestrinii (Acari: Argasidae) from Iran.
机构信息
Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
出版信息
Microb Pathog. 2020 Feb;139:103902. doi: 10.1016/j.micpath.2019.103902. Epub 2019 Nov 29.
BACKGROUND
Coxiella burnetii and non-C. burnetii bacteria or endosymbiotic Coxiella-like were reported in various tick species. We aimed to detect C. burnetii within soft tick species, Argas persicus and Alveonasus canestrinii.
METHODS
Argasid ticks were collected from different counties of Lorestan province, west of Iran. Partial fragments of 16S rRNA, IS1111 insertion sequence, com1, htpB, and icd genes related to Coxiella genus were sequenced.
RESULTS
A partial 16S rRNA and com1 gene fragment as well as IS1111 was detected in four Ar. persicus and twelve Al. canestrinii pools. Moreover, partial htpB and icd gene was only detected in one pool of Ar. persicus.
CONCLUSIONS
Detection of C. burnetii in tick samples was failed due to the occurrence of Coxiella-like endosymbionts and leads to misidentification. Thus, the house-keeping genes should be designated to distinguish C. burnetii within Coxiella-like endosymbionts.
背景
在不同种类的蜱中均有报道称存在贝纳柯克斯体（Coxiella burnetii）和非贝纳柯克斯体（非 C. burnetii）细菌或共生的柯克斯体样细菌。我们旨在检测软蜱种（Argas persicus 和 Alveonasus canestrinii）中的贝纳柯克斯体。
方法
从伊朗西部洛雷斯坦省的不同县采集到蜱。对 16S rRNA、IS1111 插入序列、com1、htpB 和 icd 基因的部分片段进行测序，这些基因与柯克斯体属有关。
结果
在 4 个 Ar. persicus 和 12 个 Al. canestrinii 池中检测到了部分 16S rRNA 和 com1 基因片段以及 IS1111。此外，仅在一个 Ar. persicus 池中检测到了部分 htpB 和 icd 基因。
结论
由于共生的柯克斯体样内共生体的存在，导致 tick 样本中贝纳柯克斯体的检测失败，从而导致了错误的鉴定。因此，应该指定管家基因来区分共生的柯克斯体样内共生体中的贝纳柯克斯体。
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