Department of Oral and Maxillofacial-Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Oral and Maxillofacial-Head & Neck Oncology, Western Central Hospital, Danzhou, China.
Oral Dis. 2020 Nov;26(8):1619-1624. doi: 10.1111/odi.13245. Epub 2020 Aug 24.
To study the role of LncRNA CASC15 in oral squamous cell carcinoma.
In the present study, we showed that plasma CASC15 was up-regulated in stage I and II oral squamous cell carcinoma patients than in oral ulcer patients and healthy controls, while no significant correlation was found between oral ulcer patients and healthy controls. Up-regulation of plasma CASC15 distinguished oral squamous cell carcinoma patients from oral ulcer patients and healthy controls. LncRNA MEG3 was inversely correlated with CASC15 in oral squamous cell carcinoma tissues. In oral squamous cell carcinoma cells, CASC15 over-expression led to the inhibited expression of MEG3, and MEG3 over-expression did not alter CASC15 expression. MEG3 over-expression decreased, while CASC15 over-expression increased the proliferation rate of oral squamous cell carcinoma cells. In addition, MEG3 over-expression attenuated the effects of CASC15 over-expression.
Therefore, CASC15 over-expression may serve as a potential diagnostic biomarker for oral squamous cell carcinoma, and CASC15 may promote oral squamous cell carcinoma cell proliferation by down-regulating MEG3.
研究 LncRNA CASC15 在口腔鳞状细胞癌中的作用。
本研究表明，与口腔溃疡患者和健康对照者相比，Ⅰ期和Ⅱ期口腔鳞状细胞癌患者的血浆 CASC15 水平升高，而口腔溃疡患者和健康对照者之间无显著相关性。血浆 CASC15 的上调将口腔鳞状细胞癌患者与口腔溃疡患者和健康对照者区分开来。在口腔鳞状细胞癌组织中，LncRNA MEG3 与 CASC15 呈负相关。在口腔鳞状细胞癌细胞中，CASC15 过表达导致 MEG3 的表达受到抑制，而 MEG3 过表达并未改变 CASC15 的表达。MEG3 过表达降低，而 CASC15 过表达则增加了口腔鳞状细胞癌细胞的增殖率。此外，MEG3 过表达减弱了 CASC15 过表达的作用。
因此，CASC15 过表达可能作为口腔鳞状细胞癌的潜在诊断生物标志物，CASC15 可能通过下调 MEG3 促进口腔鳞状细胞癌细胞的增殖。
