Susanto Agus, Satari Mieke Hemiawati, Abbas Basril, Koesoemowidodo R Setyo Adji, Cahyanto Arief
Department of Periodontics, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia.
Department of Oral Biology, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia.
Eur J Dent. 2019 Jul;13(3):370-375. doi: 10.1055/s-0039-1698610. Epub 2019 Dec 3.
The aim of this study was to evaluate the synthesis, mechanical strength, and morphology of chitosan-collagen membranes from barramundi scales for guided tissue regeneration technique.
Collagen was extracted from barramundi scales by immersion in acetic acid. The resulting wet collagen was later dried. The membrane was fabricated by mixing chitosan with collagen from barramundi scales. Membrane characterization parameters were measured using Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and mechanical property.
The FTIR spectrum showed the typical peak of the mixture of chitosan and collagen. The tensile strength and elongation at break of the membrane in dry condition were 0.28 MPa and 8.53%, respectively, while in the wet condition these were 0.12 MPa and 25.6%. The membrane porosity test result was 38.85%; SEM result showed a porous membrane surface with size varying around 16 to 100 µm.
The chitosan-collagen membrane from the barramundi scale showed the fibrous membrane surface that has ideal porous size as guided tissue regeneration membrane and the lower mechanical strength.
本研究旨在评估用于引导组织再生技术的、由尖吻鲈鳞片制备的壳聚糖 - 胶原蛋白膜的合成、机械强度和形态。
通过将尖吻鲈鳞片浸入乙酸中来提取胶原蛋白。所得的湿胶原蛋白随后进行干燥。通过将壳聚糖与来自尖吻鲈鳞片的胶原蛋白混合来制备膜。使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和机械性能来测量膜的表征参数。
FTIR光谱显示了壳聚糖和胶原蛋白混合物的典型峰。干燥条件下膜的拉伸强度和断裂伸长率分别为0.28MPa和8.53%，而在潮湿条件下分别为0.12MPa和25.6%。膜孔隙率测试结果为38.85%；SEM结果显示膜表面多孔，孔径大小在16至100μm左右变化。
由尖吻鲈鳞片制备的壳聚糖 - 胶原蛋白膜呈现出纤维状膜表面，具有作为引导组织再生膜的理想孔径大小以及较低的机械强度。
