College of Biotechnology, Sichuan University of Science and Engineering, Zigong, 643000, Sichuan province, China.
College of Biotechnology, Sichuan University of Science and Engineering, Zigong, 643000, Sichuan province, China.
Biochimie. 2021 Feb;181:1-11. doi: 10.1016/j.biochi.2020.11.016. Epub 2020 Nov 21.
Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation. C. reinhardtii has the advantages of being low cost, non-toxic, and having a post-translational modification system that ensures the recombinant proteins have the same activity as natural proteins, thus making it a great platform for application in molecular biology and other fields. In this review, we summarize the existing methods for nuclear transformation of C. reinhardtii, genes for selection, examples of foreign protein expression, and factors affecting transformation efficiency, to provide insights into effective strategies for the nuclear transformation of C. reinhardtii.
莱茵衣藻是一种模式生物，有三个已测序的基因组，能够进行基因转化。莱茵衣藻具有成本低、无毒和具有翻译后修饰系统的优点，可确保重组蛋白具有与天然蛋白相同的活性，因此成为分子生物学和其他领域应用的理想平台。在这篇综述中，我们总结了莱茵衣藻核转化的现有方法、选择基因、外源蛋白表达的实例以及影响转化效率的因素，为莱茵衣藻核转化的有效策略提供了思路。
