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并且表面活性素抑制传染性胃肠炎病毒进入肠道上皮细胞。

and surfactin inhibit the transmissible gastroenteritis virus from entering the intestinal epithelial cells.

作者信息

Wang Xiaoqing, Hu Weiwei, Zhu Liqi, Yang Qian

机构信息

College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, People's Republic of China.

College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, People's Republic of China

出版信息

Biosci Rep. 2017 Apr 10;37(2). doi: 10.1042/BSR20170082. Print 2017 Apr 28.

DOI:10.1042/BSR20170082
PMID:28270576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5469330/
Abstract

Intestinal epithelial cells are the targets for transmissible gastroenteritis (TGE) virus (TGEV) infection. It is urgent to develop a novel candidate against TGEV entry. is a probiotic with excellent anti-microorganism properties and one of its secretions, surfactin, has been regarded as a versatile weapon for most plant pathogens, especially for the enveloped virus. We demonstrate for the first time that OKB105 and its surfactin can effectively inhibit one animal coronavirus, TGEV, entering the intestinal porcine epithelial cell line (IPEC-J2). Then, several different experiments were performed to seek the might mechanisms. The plaque assays showed that surfactant could reduce the plaque generation of TGEV in a dose-dependent manner. Meanwhile, after incubation with TGEV for 1.5 h, could attach TGEV particles to their surface so that the number of virus to bind to the host cells was declined. Furthermore, our data showed that the inhibition of was closely related to the competition with TGEV for the viral entry receptors, including epidermal growth factor receptor (EGFR) and aminopeptidase N (APN) protein. In addition, Western blotting and apoptosis analysis indicated that could enhance the resistance of IPEC-J2 cells by up-regulating the expression of toll-like receptor (TLR)-6 and reducing the percentage of apoptotic cells. Taken together, our results suggest that OKB105 and its surfactin can antagonize TGEV entry and may serve as promising new candidates for TGEV prevention.

摘要

肠道上皮细胞是传染性胃肠炎（TGE）病毒（TGEV）感染的靶标。开发一种针对TGEV进入的新型候选物迫在眉睫。[具体益生菌名称未给出]是一种具有优异抗微生物特性的益生菌，其一种分泌物表面活性素被认为是对抗大多数植物病原体，尤其是包膜病毒的多功能武器。我们首次证明[具体益生菌名称未给出]OKB105及其表面活性素能有效抑制一种动物冠状病毒TGEV进入猪肠道上皮细胞系（IPEC-J2）。然后，进行了几个不同的实验来探寻可能的机制。蚀斑试验表明表面活性素能以剂量依赖的方式减少TGEV的蚀斑形成。同时，在与TGEV孵育1.5小时后，[具体益生菌名称未给出]能将TGEV颗粒附着在其表面，从而使与宿主细胞结合的病毒数量减少。此外，我们的数据表明[具体益生菌名称未给出]的抑制作用与它和TGEV竞争病毒进入受体密切相关，这些受体包括表皮生长因子受体（EGFR）和氨肽酶N（APN）蛋白。另外，蛋白质印迹法和凋亡分析表明[具体益生菌名称未给出]能通过上调Toll样受体（TLR）-6的表达并降低凋亡细胞百分比来增强IPEC-J2细胞的抗性。综上所述，我们的结果表明[具体益生菌名称未给出]OKB105及其表面活性素能拮抗TGEV进入，可能成为预防TGEV的有前景的新候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/eb9a72135c72/bsr-2017-0082i007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/0df908cb6033/bsr-2017-0082i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/9d6e43831e0d/bsr-2017-0082i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/88c14a4232fd/bsr-2017-0082i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/8347e425a5fb/bsr-2017-0082i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/a95d051c1588/bsr-2017-0082i005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/92aa5888b661/bsr-2017-0082i006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/eb9a72135c72/bsr-2017-0082i007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/0df908cb6033/bsr-2017-0082i001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/9d6e43831e0d/bsr-2017-0082i002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/88c14a4232fd/bsr-2017-0082i003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/8347e425a5fb/bsr-2017-0082i004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/a95d051c1588/bsr-2017-0082i005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/92aa5888b661/bsr-2017-0082i006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b00/5469330/eb9a72135c72/bsr-2017-0082i007.jpg

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