Park Bo Mi, Lee Jina, Park Young Kyu, Yang Young Cheol, Jung Bock Gie, Lee Bong Joo
Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
Current address: Biology Department at Morrissey College of Arts and Sciences, Boston College, Chestnut Hill, MA 02467, U.S.A.
J Poult Sci. 2023 Jul 8;60(2):2023016. doi: 10.2141/jpsa.2023016. eCollection 2023.
Benefits chitosan-fermented feed additives (CFFAs) particularly in the regulation of the immune system and antimicrobial activity. Therefore, we investigated the immune-enhancing and bacterial clearance effects of CFFA (fermented by ) on broiler chickens Gallinarum challenge. We administered 2% or 4% CFFA evaluated its immune-enhancing effects using several immunological experiments, including examination of lysozyme activity, lymphocyte proliferation, and expression of cytokines. We also evaluated the bacterial clearance effects of CFFA against Gallinarum. CFFA administration markedly enhanced lysozyme activity, lymphocyte proliferation, and the expression of interleukin (IL)-2, IL-12, tumor necrosis factor alpha, and interferon gamma in the spleen. In broilers challenged with Gallinarum, the clinical signs of Gallinarum infection and the number of viable bacterial colonies in the feces and tissues decreased in both CFFA groups. Therefore, CFFAs could be good candidates for feed additive to improve nonspecific immune responses and bacterial clearance.
壳聚糖发酵饲料添加剂（CFFAs）具有诸多益处，特别是在调节免疫系统和抗菌活性方面。因此，我们研究了CFFA（由 发酵而成）对鸡毒支原体攻毒的肉鸡的免疫增强和细菌清除作用。我们给予2%或4%的CFFA，通过多项免疫学实验评估其免疫增强效果，包括溶菌酶活性检测、淋巴细胞增殖以及细胞因子表达。我们还评估了CFFA对鸡毒支原体的细菌清除效果。给予CFFA显著增强了脾脏中的溶菌酶活性、淋巴细胞增殖以及白细胞介素（IL）-2、IL-12、肿瘤坏死因子α和干扰素γ的表达。在受到鸡毒支原体攻毒的肉鸡中，两个CFFA组的鸡毒支原体感染临床症状以及粪便和组织中活菌菌落数量均有所减少。因此，CFFAs有望成为改善非特异性免疫反应和细菌清除的优质饲料添加剂候选物。
