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从海绵中分离得到的 TM-237-S5 培养技术对次生代谢产物产生的影响。

Impact of the Cultivation Technique on the Production of Secondary Metabolites by TM-237-S5, Isolated from the Sponge .

机构信息

Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Division of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.

出版信息

Mar Drugs. 2019 Nov 30;17(12):678. doi: 10.3390/md17120678.

DOI:10.3390/md17120678
PMID:31801271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6950079/
Abstract

The fungi TM-237-S5 was isolated from the sponge , collected from the mesophotic coral ecosystem of the Red Sea. The strain was cultivated on a potato dextrose agar (PDA) medium, coupling solid-state fermentation and solid-state extraction (SSF/SSE) with a neutral macroreticular polymeric adsorbent XAD Amberlite resin (AMBERLITE XAD1600N). The SSF/SSE lead to high chemodiversity and productivity compared to classical submerged cultivation. Ten phenalenone related compounds were isolated and fully characterized by one-dimensional and two-dimensional NMR and HRMS. Among them, four were found to be new compounds corresponding to isoconiolactone, (-)-peniciphenalenin F, (+)-8-hydroxyscleroderodin, and (+)-8-hydroxysclerodin. It is concluded that SSF/SSE is a powerful strategy, opening a new era for the exploitation of microbial secondary metabolites.

摘要

从红海中层珊瑚礁生态系统中采集的海绵中分离出真菌 TM-237-S5。该菌株在马铃薯葡萄糖琼脂（PDA）培养基上培养，采用固态发酵和固态萃取（SSF/SSE）与中性大孔聚合物吸附剂 XAD Amberlite 树脂（AMBERLITE XAD1600N）相结合。与传统的液体深层培养相比，SSF/SSE 导致了更高的化学多样性和生产力。分离并通过一维和二维 NMR 和高分辨率质谱（HRMS）对 10 种与 phenalenone 相关的化合物进行了充分的表征。其中，发现有 4 种是新化合物，分别为 isoconiolactone、（-）-peniciphenalenin F、（+）-8-羟基硬脂酸和（+）-8-羟基硬脂酸。可以得出结论，SSF/SSE 是一种强大的策略，为微生物次生代谢产物的开发开辟了一个新时代。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/bd078f61f6f0/marinedrugs-17-00678-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/9ab80f4984ea/marinedrugs-17-00678-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/f9455a0eac4f/marinedrugs-17-00678-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/44544518aa2d/marinedrugs-17-00678-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/11bcaca66c8b/marinedrugs-17-00678-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/23450bc1c98f/marinedrugs-17-00678-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/6e6ef899b6b7/marinedrugs-17-00678-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/32bc4d66274f/marinedrugs-17-00678-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/6fe761f8067d/marinedrugs-17-00678-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/bd078f61f6f0/marinedrugs-17-00678-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/9ab80f4984ea/marinedrugs-17-00678-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/f9455a0eac4f/marinedrugs-17-00678-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/44544518aa2d/marinedrugs-17-00678-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/11bcaca66c8b/marinedrugs-17-00678-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/23450bc1c98f/marinedrugs-17-00678-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/6e6ef899b6b7/marinedrugs-17-00678-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/32bc4d66274f/marinedrugs-17-00678-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/6fe761f8067d/marinedrugs-17-00678-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a37/6950079/bd078f61f6f0/marinedrugs-17-00678-g009.jpg

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