Wang Tingting, Zhou Jing, Zou Jiabin, Shi Yutong, Zhou Wenli, Shao Peng, Yu Tianze, Cui Wei, Li Xiaohui, Wu Xingxin, Ye Jing, Yan Xiaojun, Naman C Benjamin, Lazaro J Enrico H, He Shan
Li Dak Sum Marine Biopharmaceutical Research Center, Department of Marine Pharmacy, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China.
College of Fisheries, Tianjin Agricultural University, Tianjin, China.
Front Microbiol. 2021 Feb 22;12:638610. doi: 10.3389/fmicb.2021.638610. eCollection 2021.
Mesophotic coral ecosystems (MCEs) have complex but understudied biodiversity, especially for natural products discovery. Untargeted metabolomics research on 80 extracts prepared from marine sponge-associated fungi, half from shallow reefs (<30 m) and half from MCEs (30-150 m), facilitated prioritization for further study a fungus from a 103 m deep sponge. LC-MS target-directed isolation yielded a series of new compounds, cymopolyphenols A-F (-), and two known phenylspirodrimanes, F1839-I () and stachybotrylactone (). This is the first report of natural products from the recently described genus, . Compounds - and contain a dihydroisobenzofuran moiety, and - are low-order polymers of with novel scaffolds. The structures of the compounds were established by spectroscopic and spectrometric data interpretation, with further support from X-ray crystallography studies of and . Compound undergoes facile racemization in solution and was found to crystalize as a racemic mixture. Compound was also obtained in racemic form, and after chiral chromatography, both separated enantiomers racemized in solution by a presumed keto-enol tautomerization. Compounds and - were found to be weakly antimicrobial (MIC 16-64 μg/ml) against several Gram-positive and Gram-negative human or aquatic pathogens, compound was shown to chelate iron at 10 μM, and activated plant disease resistance in a transgenic model organism.
中光层珊瑚生态系统（MCEs）具有复杂但研究不足的生物多样性，尤其是在天然产物发现方面。对从与海洋海绵相关的真菌中制备的80种提取物进行非靶向代谢组学研究，其中一半来自浅礁（<30米），一半来自中光层珊瑚生态系统（30 - 150米），有助于对来自103米深处海绵的一种真菌进行进一步研究的优先级排序。液相色谱 - 质谱靶向分离产生了一系列新化合物，即环多酚A - F（-），以及两种已知的苯基螺二氢萘，F1839 - I（）和葡萄穗霉内酯（）。这是最近描述的属中天然产物的首次报道。化合物 - 和 含有二氢异苯并呋喃部分，而 - 是具有新型支架的 的低阶聚合物。通过光谱和光谱数据解释确定了化合物的结构，并得到了 和 的X射线晶体学研究的进一步支持。化合物 在溶液中容易发生外消旋化，并且被发现以外消旋混合物形式结晶。化合物 也以外消旋形式获得，在手性色谱后，两种分离的对映体通过假定的酮 - 烯醇互变异构在溶液中外消旋化。发现化合物 和 - 对几种革兰氏阳性和革兰氏阴性人类或水生病原体具有弱抗菌活性（MIC为16 - 64μg/ml），化合物 在10μM时显示出螯合铁的能力，并且 在转基因模式生物中激活了植物抗病性。
