Magnotti R A, Dowling K, Eberly J P, McConnell R S
University of Cincinnati Department of Internal Medicine, OH.
Clin Chim Acta. 1988 Sep 15;176(3):315-32. doi: 10.1016/0009-8981(88)90190-8.
We have optimized Ellman-type plasma and erythrocyte cholinesterase assays for the field determination of pesticide exposure. The assays employ stable, premixed, inexpensive colorimetric reagents and a simplified kinetic measurement procedure using a novel battery-operable light-emitting-diode-source colorimeter (LEDSC). These methods were compared to a field tintometric method for cholinesterase currently in widespread use, using samples from organophosphate-intoxicated hospitalized patients. In comparison to the colorimetric method, the tintometric method was less precise and consistently underestimated the extent of pesticide exposure. Methods for the effective field determination of pesticide exposure using cholinesterase assays are critically evaluated with regard to their practical, biochemical, and medical relevance.
我们已经优化了用于现场测定农药暴露的埃尔曼型血浆和红细胞胆碱酯酶检测方法。这些检测采用稳定、预混、廉价的比色试剂，并使用一种新型的电池供电发光二极管源比色计（LEDSC）进行简化的动力学测量程序。使用来自有机磷中毒住院患者的样本，将这些方法与目前广泛使用的现场比色法测定胆碱酯酶进行了比较。与比色法相比，比色法的精度较低，并且一直低估了农药暴露的程度。使用胆碱酯酶检测方法进行农药暴露有效现场测定的方法，在实际、生化和医学相关性方面受到了严格评估。
