一种用于评估维生素 B6 状况的荧光腙交换探针吡哆醛磷酸盐。
A fluorescent hydrazone exchange probe of pyridoxal phosphate for the assessment of vitamin B6 status.
机构信息
Department of Chemistry and ChEM-H Institute, Stanford University, Stanford, CA 94305, USA.
出版信息
Chem Commun (Camb). 2019 Dec 19;56(2):317-320. doi: 10.1039/c9cc08458d.
Abstract
Abnormal vitamin B6 status, marked by deficient intracellular concentrations of pyridoxal phosphate (PLP), is classified as a direct biomarker based on its biomedical significance. However, there exist no direct methods for measuring vitamin B6 status in intact cells. Here we describe the development of a fluorogenic probe, RAB6, which shows remarkable selectivity for PLP among the B6 vitamers and other cellular aldehydes.
摘要
异常的维生素 B6 状态，表现为细胞内吡哆醛 5-磷酸（PLP）浓度不足，根据其生物医学意义被归类为直接生物标志物。然而，目前还没有直接测量完整细胞中维生素 B6 状态的方法。在这里，我们描述了一种荧光探针 RAB6 的开发，该探针对 B6 维生素和其他细胞醛具有显著的选择性。
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