Kuang Xu, Zhang Yan, Liu Juan, Yang Run-Shi, Qiu Zhi-Ying, Sun Jian, Liao Xiao-Ping, Liu Ya-Hong, Yu Yang
National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2022 Jul 1;13:912260. doi: 10.3389/fmicb.2022.912260. eCollection 2022.
We conducted a molecular surveillance study for carbapenem-resistant (CRE) colonization in food-producing animals in China that included primarily swine and poultry for three consecutive years. A total of 2,771 samples from food-producing animals and their surrounding environments were collected from different regions in China from 2015 to 2017. Enrichment cultures supplemented with meropenem were used to isolate carbapenem non-susceptible isolates and these were subsequently identified by MALDI-TOF MS. Resistance phenotypes and genotypes were confirmed using antimicrobial susceptibility testing and molecular biological techniques. Genomic characteristics of the carbapenemase-producing isolates were investigated using whole genome sequencing (WGS) and bioinformatic analysis. In total, 88 NDM-positive were identified from 2,771 samples and 96.6% were . The New Delhi metallo--lactamase (NDM)-positive displayed a diversity of sequence types (ST), and ST48 and ST165 were the most prevalent. Three variants of ( , , and ) were detected and WGS indicated that predominated and was carried primarily on IncX3 plasmids. All these isolates were also multiply-drug resistant. These results revealed that food-producing animals in China are an important reservoir for NDM-positive and pose a potential threat to public health.
我们在中国开展了一项针对食品生产动物耐碳青霉烯类肠杆菌科细菌（CRE）定植情况的分子监测研究，该研究连续三年主要纳入了猪和家禽。2015年至2017年期间，从中国不同地区采集了共计2771份来自食品生产动物及其周边环境的样本。使用添加美罗培南的增菌培养物来分离对碳青霉烯类不敏感的菌株，随后通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对这些菌株进行鉴定。通过抗菌药物敏感性测试和分子生物学技术来确认耐药表型和基因型。利用全基因组测序（WGS）和生物信息学分析对产碳青霉烯酶菌株的基因组特征进行了研究。在2771份样本中总共鉴定出了来自食品生产动物及其周边环境的样本。使用添加美罗培南的增菌培养物来分离对碳青霉烯类不敏感的菌株，随后通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对这些菌株进行鉴定。通过抗菌药物敏感性测试和分子生物学技术来确认耐药表型和基因型。利用全基因组测序（WGS）和生物信息学分析对产碳青霉烯酶菌株的基因组特征进行了研究。在2771份样本中总共鉴定出88株新德里金属β-内酰胺酶（NDM）阳性菌株，其中96.6%为大肠杆菌。NDM阳性大肠杆菌呈现出多种序列类型（ST），其中ST48和ST165最为常见。检测到了三种blaNDM变体（blaNDM-1、blaNDM-5和blaNDM-9），全基因组测序表明blaNDM-1占主导地位，并且主要携带在IncX3质粒上。所有这些菌株还对多种药物耐药。这些结果表明，中国的食品生产动物是NDM阳性大肠杆菌的重要储存宿主，对公众健康构成潜在威胁。
