National Reference Centre for Poultry and Rabbit Diseases (NRGK), Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
PLoS One. 2019 Dec 10;14(12):e0226091. doi: 10.1371/journal.pone.0226091. eCollection 2019.
In Switzerland, domestic turkey meat is a niche product. Turkeys are fattened on mixed family-based farms scattered across the country, with most providing access to an uncovered outdoor pasture for the birds. Swiss fattening turkeys may therefore get infected with Chlamydiaceae via wild birds or their faeces, potentially shedding these bacteria at a later stage. The aim of the present study was to acquire baseline data about the shedding of Chlamydiaceae in clinically unremarkable Swiss fattening turkeys at slaughter, potentially exposing slaughterhouse workers to infection. In this large-scale study, 1008 cloacal swabs of Swiss turkeys out of 53 flocks from 28 different grow-out farms with uncovered outdoor pasture were collected over the course of 14 months and examined for the occurrence of Chlamydiaceae by a family-specific 23S-rRNA real-time PCR. Positive samples were further analyzed by Chlamydia psittaci (C. psittaci)-specific real-time PCR and the Arraymate DNA Microarray for species identification. All samples were negative for C. psittaci, but seven swabs out of one flock were tested positive for Chlamydia gallinacea (0.7%). Although turkeys with access to pasture may have contact with Chlamydiaceae-harbouring wild birds or their faeces, the infection rate in Swiss turkeys was shown to be low.
在瑞士，国产火鸡肉是一种小众产品。火鸡在全国各地的混合家庭农场中育肥，大多数农场为鸟类提供了一个无遮盖的户外牧场。因此，瑞士育肥火鸡可能会通过野鸟或它们的粪便感染衣原体科，在以后的某个阶段有可能会排出这些细菌。本研究的目的是在屠宰时获得有关临床无明显症状的瑞士育肥火鸡中衣原体科脱落的基线数据，这可能会使屠宰场工人面临感染的风险。在这项大规模研究中，在 14 个月的时间里，从 28 个不同的户外牧场饲养农场的 53 个批次中采集了 1008 份瑞士火鸡的泄殖腔拭子，并通过针对特定家族的 23S-rRNA 实时 PCR 检测衣原体科的存在。对阳性样本进一步进行鹦鹉热衣原体（C. psittaci）特异性实时 PCR 和 Arraymate DNA 微阵列分析以确定物种。所有样本均为阴性，没有检测到鹦鹉热衣原体，但是一个批次的七个拭子检测出了鸡衣原体（0.7%）。尽管有牧场的火鸡可能与携带衣原体科的野鸟或它们的粪便接触，但瑞士火鸡的感染率较低。
