Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.
Jill Roberts Institute for Research in Inflammatory Bowel Disease, Department of Medicine, Weill Cornell Medicine, NY 10021, USA.
Science. 2019 Dec 13;366(6471). doi: 10.1126/science.aav1282.
The gut microbiota produce hundreds of molecules that are present at high concentrations in the host circulation. Unraveling the contribution of each molecule to host biology remains difficult. We developed a system for constructing clean deletions in spp., the source of many molecules from the gut microbiome. By applying this method to the model commensal organism , we knocked out genes for 10 -derived molecules that accumulate in host tissues. In mice colonized by a for which the production of branched short-chain fatty acids was knocked out, we discovered that these microbial products have immunoglobulin A-modulatory activity.
肠道微生物群产生数百种分子，这些分子在宿主循环中以高浓度存在。揭示每种分子对宿主生物学的贡献仍然很困难。我们开发了一种在 spp.中构建清洁缺失的系统，该菌是肠道微生物组中许多分子的来源。通过将这种方法应用于模型共生体 ，我们敲除了在宿主组织中积累的 10 种衍生分子的基因。在被敲除支链短链脂肪酸产生的 定植的小鼠中，我们发现这些微生物产物具有调节免疫球蛋白 A 的活性。
