Suppr超能文献

利用遗传学耗尽宿主来源的微生物组分子。

Depletion of microbiome-derived molecules in the host using genetics.

机构信息

Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.

Jill Roberts Institute for Research in Inflammatory Bowel Disease, Department of Medicine, Weill Cornell Medicine, NY 10021, USA.

出版信息

Science. 2019 Dec 13;366(6471). doi: 10.1126/science.aav1282.

Abstract

The gut microbiota produce hundreds of molecules that are present at high concentrations in the host circulation. Unraveling the contribution of each molecule to host biology remains difficult. We developed a system for constructing clean deletions in spp., the source of many molecules from the gut microbiome. By applying this method to the model commensal organism , we knocked out genes for 10 -derived molecules that accumulate in host tissues. In mice colonized by a for which the production of branched short-chain fatty acids was knocked out, we discovered that these microbial products have immunoglobulin A-modulatory activity.

摘要

肠道微生物群产生数百种分子,这些分子在宿主循环中以高浓度存在。揭示每种分子对宿主生物学的贡献仍然很困难。我们开发了一种在 spp.中构建清洁缺失的系统,该菌是肠道微生物组中许多分子的来源。通过将这种方法应用于模型共生体 ,我们敲除了在宿主组织中积累的 10 种衍生分子的基因。在被敲除支链短链脂肪酸产生的 定植的小鼠中,我们发现这些微生物产物具有调节免疫球蛋白 A 的活性。

相似文献

1
Depletion of microbiome-derived molecules in the host using genetics.
Science. 2019 Dec 13;366(6471). doi: 10.1126/science.aav1282.
2
Modulating microbiome metabolites in vivo.
Science. 2020 Jul 10;369(6500):153. doi: 10.1126/science.abc5620.
3
A metabolic pathway for bile acid dehydroxylation by the gut microbiome.
Nature. 2020 Jun;582(7813):566-570. doi: 10.1038/s41586-020-2396-4. Epub 2020 Jun 17.
4
Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.
Cell. 2022 Feb 3;185(3):547-562.e22. doi: 10.1016/j.cell.2021.12.035. Epub 2022 Jan 19.
5
increases fat accumulation in mice by enhancing energy absorption and adipogenesis.
Microbiol Spectr. 2024 Aug 6;12(8):e0411623. doi: 10.1128/spectrum.04116-23. Epub 2024 Jun 25.
7
CRISPR Genome Editing Systems in the Genus : a Timely Advancement.
J Bacteriol. 2019 Jul 24;201(16). doi: 10.1128/JB.00219-19. Print 2019 Aug 15.
8
A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites.
Nature. 2017 Nov 30;551(7682):648-652. doi: 10.1038/nature24661. Epub 2017 Nov 22.
9
CRISPR-Cas12a-Mediated Gene Deletion and Regulation in and Its Application in Carbon Flux Redirection in Synthesis Gas Fermentation.
ACS Synth Biol. 2019 Oct 18;8(10):2270-2279. doi: 10.1021/acssynbio.9b00033. Epub 2019 Sep 26.
10
Gene editing and genetic engineering approaches for advanced probiotics: A review.
Crit Rev Food Sci Nutr. 2018 Jul 3;58(10):1735-1746. doi: 10.1080/10408398.2016.1274877. Epub 2017 Jul 21.

引用本文的文献

1
Straining to define a healthy microbiome.
mSphere. 2025 Jul 29;10(7):e0079724. doi: 10.1128/msphere.00797-24. Epub 2025 Jul 11.
2
Microbiota-dependent metabolites - New engine for T cell warriors.
Gut Microbes. 2025 Dec;17(1):2523815. doi: 10.1080/19490976.2025.2523815. Epub 2025 Jun 30.
3
The functional landscape of the appendix microbiome under conditions of health and disease.
Gut Pathog. 2025 Jun 1;17(1):38. doi: 10.1186/s13099-025-00696-2.
5
Microbiota mechanisms in cancer progression and therapy.
Cell Chem Biol. 2025 May 15;32(5):653-677. doi: 10.1016/j.chembiol.2025.04.005. Epub 2025 May 6.
6
Population imaging of enterochromaffin cell activity reveals regulation by somatostatin.
Proc Natl Acad Sci U S A. 2025 May 13;122(19):e2501525122. doi: 10.1073/pnas.2501525122. Epub 2025 May 6.
8
Gut Microbiota Regulate Saturated Free Fatty Acid Metabolism in Heart Failure.
Small Sci. 2024 Jul 8;4(9):2300337. doi: 10.1002/smsc.202300337. eCollection 2024 Sep.
9
Conserved genetic basis for microbial colonization of the gut.
Cell. 2025 May 1;188(9):2505-2520.e22. doi: 10.1016/j.cell.2025.03.010. Epub 2025 Apr 4.
10
Human milk oligosaccharides: bridging the gap in intestinal microbiota between mothers and infants.
Front Cell Infect Microbiol. 2025 Jan 6;14:1386421. doi: 10.3389/fcimb.2024.1386421. eCollection 2024.

本文引用的文献

1
Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes.
Science. 2018 Mar 9;359(6380):1151-1156. doi: 10.1126/science.aao5774.
2
A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites.
Nature. 2017 Nov 30;551(7682):648-652. doi: 10.1038/nature24661. Epub 2017 Nov 22.
3
Metabolic, Epigenetic, and Transgenerational Effects of Gut Bacterial Choline Consumption.
Cell Host Microbe. 2017 Sep 13;22(3):279-290.e7. doi: 10.1016/j.chom.2017.07.021. Epub 2017 Aug 24.
4
Enterochromaffin Cells Are Gut Chemosensors that Couple to Sensory Neural Pathways.
Cell. 2017 Jun 29;170(1):185-198.e16. doi: 10.1016/j.cell.2017.05.034. Epub 2017 Jun 22.
5
Modulation of a Circulating Uremic Solute via Rational Genetic Manipulation of the Gut Microbiota.
Cell Host Microbe. 2016 Dec 14;20(6):709-715. doi: 10.1016/j.chom.2016.10.021. Epub 2016 Dec 1.
6
Gut microbiota, metabolites and host immunity.
Nat Rev Immunol. 2016 May 27;16(6):341-52. doi: 10.1038/nri.2016.42.
7
Consequences of bile salt biotransformations by intestinal bacteria.
Gut Microbes. 2016;7(1):22-39. doi: 10.1080/19490976.2015.1127483.
8
High-Specificity Targeted Functional Profiling in Microbial Communities with ShortBRED.
PLoS Comput Biol. 2015 Dec 18;11(12):e1004557. doi: 10.1371/journal.pcbi.1004557. eCollection 2015 Dec.
9
MetaQuery: a web server for rapid annotation and quantitative analysis of specific genes in the human gut microbiome.
Bioinformatics. 2015 Oct 15;31(20):3368-70. doi: 10.1093/bioinformatics/btv382. Epub 2015 Jun 22.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验