中国一种多年生草本植物16个单拷贝核基因标记的开发。
Development of 16 single-copy nuclear gene markers for , a perennial herb in China.
作者信息
Sun Zhixia, Feng Chao, Yang Lihua, Kong Hanghui
机构信息
Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou People's Republic of China.
College of Life Sciences University of Chinese Academy of Sciences Beijing People's Republic of China.
出版信息
Appl Plant Sci. 2019 Nov 14;7(11):e11304. doi: 10.1002/aps3.11304. eCollection 2019 Nov.
PREMISE
We developed single-copy nuclear DNA markers for a perennial herb, (Gesneriaceae), to help infer the evolutionary history of the genus in subtropical China.
METHODS AND RESULTS
We screened 834 putative single-copy nuclear genes from transcriptomes of 11 species of using the Illumina HiSeq 2000 platform. Based on the screening results, we developed 16 primer sets for accessions of representing three wild populations. The number of alleles per locus ranged from three to 34. The levels of expected and observed heterozygosity varied from 0.000 to 0.372 and 0.000 to 0.650, respectively. The markers were successfully cross-amplified in the related species , , , and .
CONCLUSIONS
The newly developed markers will facilitate further studies on genetic diversity and phylogeographic structure throughout the distributional range of . Additionally, these markers may be useful for other related species in Gesneriaceae.
前提
我们为一种多年生草本植物（苦苣苔科）开发了单拷贝核DNA标记，以帮助推断中国亚热带地区该属的进化历史。
方法与结果
我们使用Illumina HiSeq 2000平台，从11种植物的转录组中筛选了834个假定的单拷贝核基因。基于筛选结果，我们为代表三个野生种群的植物样本开发了16对引物。每个位点的等位基因数量从3到34不等。预期杂合度和观察到的杂合度水平分别在0.000至0.372和0.000至0.650之间变化。这些标记在相关物种、、和中成功进行了交叉扩增。
结论
新开发的标记将有助于进一步研究该植物在整个分布范围内的遗传多样性和系统地理学结构。此外，这些标记可能对苦苣苔科的其他相关物种有用。
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