苜蓿同源域手指蛋白 MtPHD6 的异位表达增强拟南芥的抗旱性。
Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis.
机构信息
Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan, Hubei, China.
Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Bonn, Germany.
出版信息
BMC Genomics. 2019 Dec 16;20(1):982. doi: 10.1186/s12864-019-6350-5.
BACKGROUND
The plant homeodomain (PHD) finger is a CysHisCys-type zinc finger which promotes protein-protein interactions and binds to the cis-acting elements in the promoter regions of target genes. In Medicago truncatula, five PHD homologues with full-length sequence were identified. However, the detailed function of PHD genes was not fully addressed.
RESULTS
In this study, we characterized the function of MtPHD6 during plant responses to drought stress. MtPHD6 was highly induced by drought stress. Ectopic expression of MtPHD6 in Arabidopsis enhanced tolerance to osmotic and drought stresses. MtPHD6 transgenic plants exhibited decreased water loss rate, MDA and ROS contents, and increased leaf water content and antioxidant enzyme activities under drought condition. Global transcriptomic analysis revealed that MtPHD6 reprogramed transcriptional networks in transgenic plants. Expression levels of ABA receptor PYR/PYLs, ZINC FINGER, AP2/EREBP and WRKY transcription factors were mainly up-regulated after transformation of MtPHD6. Interaction network analysis showed that ZINC FINGER, AP2/EREBP and WRKY interacted with each other and downstream stress induced proteins.
CONCLUSIONS
We proposed that ZINC FINGER, AP2/EREBP and WRKY transcription factors were activated through ABA dependent and independent pathways to increase drought tolerance of MtPHD6 transgenic plants.
背景
植物同源结构域（PHD）指是一种 CysHisCys 型锌指，能够促进蛋白质-蛋白质相互作用，并与靶基因启动子区域中的顺式作用元件结合。在蒺藜苜蓿中，鉴定到了具有全长序列的五个 PHD 同源物。然而，PHD 基因的详细功能尚未完全阐明。
结果
在这项研究中，我们研究了 MtPHD6 在植物响应干旱胁迫过程中的功能。MtPHD6 受干旱胁迫诱导表达。在拟南芥中异位表达 MtPHD6 增强了其对渗透和干旱胁迫的耐受性。在干旱条件下，MtPHD6 转基因植物的水分损失率、MDA 和 ROS 含量降低，叶片含水量和抗氧化酶活性增加。全转录组分析表明，MtPHD6 重塑了转基因植物的转录网络。ABA 受体 PYR/PYLs、ZINC FINGER、AP2/EREBP 和 WRKY 转录因子的表达水平在转化 MtPHD6 后主要上调。互作网络分析表明，ZINC FINGER、AP2/EREBP 和 WRKY 转录因子相互作用，并与下游诱导的胁迫蛋白相互作用。
结论
我们提出 ZINC FINGER、AP2/EREBP 和 WRKY 转录因子通过 ABA 依赖和非依赖途径被激活，从而提高 MtPHD6 转基因植物的抗旱性。
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