a School of Food and Biological Engineering , Jiangsu University , Zhenjiang , China.
Int J Food Sci Nutr. 2019 May;70(3):303-310. doi: 10.1080/09637486.2018.1511687. Epub 2018 Sep 24.
We have previously reported an aqueous extract of fermented barley with Lactobacillus plantarum dy-1 (LFBE) has more efficient anti-obesity effect compared with that of Saccharomyces cerevisiae. To further explore associated effects of LFBE on body weight and body fat distribution, and lipid profiles related metabolic outcomes, serum metabolites were analysed using LC-MS-MS and partial least-squares-discriminant analysis (PLS-DA). Obese and lean groups were clearly discriminated from each other on PLS-DA score plot and major metabolites contributing to the discrimination were assigned as lipid metabolites (fatty acids), lipid metabolism intermediates (choline, betaine, carnitine and butyryl-carnitine), amino acids and citric acid. A high-fat diet increased lipid metabolites and decreased lipid metabolism intermediates, indicating that abnormal lipid metabolism induced by a high-fat diet resulted in fat accumulation via decreased β-oxidation. But LFBE can inhibit fat accumulation by reducing lipid metabolites and increasing lipid metabolism intermediates. Furthermore, the level changes of these metabolites can be used to assess the risk of obesity and the therapeutic effect of obesity management.
我们之前报道过，与酿酒酵母相比，植物乳杆菌 dy-1（LFBE）发酵大麦的水提物具有更有效的抗肥胖作用。为了进一步探讨 LFBE 对体重和体脂分布的相关影响，以及与脂质代谢相关的代谢结果，我们使用 LC-MS-MS 和偏最小二乘判别分析（PLS-DA）分析了血清代谢物。PLS-DA 得分图清楚地区分了肥胖组和瘦重组，导致这种区分的主要代谢物被分配为脂质代谢物（脂肪酸）、脂质代谢中间产物（胆碱、甜菜碱、肉碱和丁酰肉碱）、氨基酸和柠檬酸。高脂肪饮食增加了脂质代谢物，减少了脂质代谢中间产物，表明高脂肪饮食引起的异常脂质代谢通过减少β-氧化导致脂肪堆积。但 LFBE 可以通过减少脂质代谢物和增加脂质代谢中间产物来抑制脂肪堆积。此外，这些代谢物的水平变化可用于评估肥胖风险和肥胖管理的治疗效果。
