He Yining, Huang Yun, Zhang Zhengyin, Yu Fengping, Zheng Yingjie
Department of Epidemiology, School of Public Health, Fudan University, Shanghai, China.
Department of Clinical Laboratory, Shanghai Punan Hospital of Pudong New District, Shanghai, China.
PeerJ. 2019 Dec 10;7:e8172. doi: 10.7717/peerj.8172. eCollection 2019.
This study was designed to explore the profile and potential influencers of the vaginal microbiome (VMB) among asymptomatic pregnant Chinese women and its possible association with pregnancy outcomes.
A prospective study was conducted among pregnant Chinese women receiving regular prenatal care at a hospital in Shanghai, China from March 2017 to March 2018. Vaginal swabs were obtained from 113 asymptomatic pregnant women in mid-pregnancy and sequenced by the V3-V4 region of 16S rRNA on an Ion S5™ XL platform. Demographic characteristics and major pregnancy outcomes were collected through questionnaires and electronic medical records.
The predominant vaginal community state types (CSTs) were CST I (45.1%) and CST III (31.9%). Participants were divided into a lactobacilli-dominant group (LD, CST I/II/III/I-III/V, = 100, 88.5%) and a less lactobacilli-dominant group (LLD, CST IV-A/B, = 13, 11.5%). Women in the LLD group showed an increased alpha diversity [median (interquartile range, IQR): 2.41 (1.67, 2.49) vs. 0.30 (0.17, 0.59), < 0.001], which was related to a lower pre-pregnancy body mass index (BMI) ( = 0.012), and a greater instance of passive smoking ( = 0.033). The relative abundance of was correlated positively with the pre-pregnancy BMI ( = 0.177, = 0.041), but negatively with passive smoking ( = - 0.204, = 0.030).
The vaginal flora of asymptomatic pregnant Chinese women was mostly dominated by and . A lower BMI and greater instance of passive smoking may contribute to a less lactobacilli-dominant VMB. However, a larger sample size is needed.
本研究旨在探讨无症状中国孕妇阴道微生物群（VMB）的特征、潜在影响因素及其与妊娠结局的可能关联。
2017年3月至2018年3月，在中国上海一家医院对接受定期产前检查的中国孕妇进行了一项前瞻性研究。从113名孕中期无症状孕妇中获取阴道拭子，并在Ion S5™ XL平台上对16S rRNA的V3-V4区域进行测序。通过问卷调查和电子病历收集人口统计学特征和主要妊娠结局。
主要的阴道群落状态类型（CSTs）为CST I（45.1%）和CST III（31.9%）。参与者被分为乳酸菌占主导的组（LD，CST I/II/III/I-III/V，n = 100，88.5%）和乳酸菌占比少的组（LLD，CST IV-A/B，n = 13，11.5%）。LLD组女性的α多样性增加[中位数（四分位间距，IQR）：2.41（1.67，2.49）对0.30（0.17，0.59），P < 0.001]，这与孕前较低的体重指数（BMI）（P = 0.012）和更多的被动吸烟情况（P = 0.033）有关。[某种菌]的相对丰度与孕前BMI呈正相关（P = 0.177，r = 0.041），但与被动吸烟呈负相关（P = -0.204，r = 0.030）。
无症状中国孕妇的阴道菌群大多以[两种菌]为主。较低的BMI和更多的被动吸烟情况可能导致乳酸菌占主导的VMB减少。然而，需要更大的样本量。
