Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
Clinical Laboratory, Huadong Hospital of Fudan University, Shanghai, China.
Front Cell Infect Microbiol. 2021 Aug 19;11:680643. doi: 10.3389/fcimb.2021.680643. eCollection 2021.
The role of vaginal microbiota in recurrent spontaneous abortion (RSA) remains unknown. The purpose of this study was to investigate characteristics of vaginal microbiota and the effects of drug treatment on vaginal microbiota of patients with RSA.
A case-control study was performed, in which non-pregnant patients who experienced RSA were selected and divided into untreated and drug-treated groups. Drug-treated patients were subdivided into the metformin group, metformin plus aspirin group, and other drugs group. Healthy women who had live births and never experienced spontaneous abortion were enrolled in the control group. Characteristics of vaginal microbiomes of patients with RSA and healthy women and the impact of drug treatment on the microbiome was evaluated 16S rRNA gene sequencing of the V3-V4 region using the Illumina MiSeq platform.
Women who underwent RSA had lower microbial richness than healthy women. Compared to controls, the relative abundance of seven taxa (, , -, and _) in the patient's vaginal microbiota changed significantly, which may be closely related to RSA. The composition of the vaginal microbial community in RSA patients was altered by drug treatment. Metformin combined with aspirin treatment significantly increased the relative abundance of vaginal spp. in patients.
An altered vaginal microbiome composition might be associated with RSA, which could be modified by drug treatment. The effect of metformin combined with aspirin on vaginal is worthy of attention.
阴道微生物群在复发性自然流产（RSA）中的作用尚不清楚。本研究旨在探讨阴道微生物群的特征以及药物治疗对 RSA 患者阴道微生物群的影响。
采用病例对照研究，选择经历 RSA 的非妊娠患者，并将其分为未治疗组和药物治疗组。药物治疗组患者进一步分为二甲双胍组、二甲双胍联合阿司匹林组和其他药物组。将有活产且从未经历过自然流产的健康妇女纳入对照组。使用 Illumina MiSeq 平台对 V3-V4 区的 16S rRNA 基因进行测序，评估 RSA 患者和健康妇女的阴道微生物组特征，以及药物治疗对微生物组的影响。
经历 RSA 的女性的微生物丰富度低于健康女性。与对照组相比，患者阴道微生物群中七种分类群（，，，和_）的相对丰度发生了显著变化，这可能与 RSA 密切相关。RSA 患者阴道微生物群落的组成通过药物治疗发生了改变。二甲双胍联合阿司匹林治疗显著增加了患者阴道内 spp.的相对丰度。
改变的阴道微生物组组成可能与 RSA 相关，药物治疗可能会改变其组成。二甲双胍联合阿司匹林对阴道内 的影响值得关注。
