Gold nanoparticles with special surface plasmon resonance have been widely used in sensing and therapy because of their easy preparation, unique optical properties, excellent biocompatibility, etc. The applications of gold nanoparticles in chemo/biosensing, imaging, and therapy reported in 2016-2019, are summarized in this review. Regarding the gold nanoparticle-based sensing or imaging, sensing mechanisms and strategies are provided to illustrate the concepts for designing sensitive and selective detection platforms. Gold nanoparticlemediated therapy is introduced by surface plasmon resonance-based therapy and delivery-based therapy. Beyond the sole therapeutic system, platforms through synergistic therapy are also discussed. In the end, discussion of the challenges and future trends of gold nanoparticle-based sensing and therapy systems is described.