Department of Vasculocardiology, The Affiliated Hospital of Southwest Medical University, Key Laboratory of Medical Electrophysiology, Ministry of Education, Southwest Medical University, Luzhou, Sichuan, 646000, China.
Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan, 646000, China.
Small. 2021 Feb;17(8):e2004287. doi: 10.1002/smll.202004287. Epub 2021 Feb 1.
With the advent of nanofabrication techniques, plasmonic nanoparticles (PNPs) have been widely applied in various research fields ranging from photocatalysis to chemical and bio-sensing. PNPs efficiently convert chemical or physical stimuli in their local environment into optical signals. PNPs also have excellent properties, including good biocompatibility, large surfaces for the attachment of biomolecules, tunable optical properties, strong and stable scattering light, and good conductivity. Thus, single optical biosensors with plasmonic properties enable a broad range of uses of optical imaging techniques in biological sensing and imaging with high spatial and temporal resolution. This work provides a comprehensive overview on the optical properties of single PNPs, the description of five types of commonly used optical imaging techniques, including surface plasmon resonance (SPR) microscopy, surface-enhanced Raman scattering (SERS) technique, differential interference contrast (DIC) microscopy, total internal reflection scattering (TIRS) microscopy, and dark-field microscopy (DFM) technique, with an emphasis on their single plasmonic nanoprobes and mechanisms for applications in biological imaging and sensing, as well as the challenges and future trends of these fields.
随着纳米制造技术的出现，等离子体纳米粒子（PNP）已经被广泛应用于从光催化到化学和生物传感等各个研究领域。PNP 能够将其局部环境中的化学或物理刺激有效地转化为光学信号。PNP 还具有出色的性能，包括良好的生物相容性、用于附着生物分子的大表面、可调光学特性、强而稳定的散射光以及良好的导电性。因此，具有等离子体特性的单个光学生物传感器能够在具有高时空分辨率的生物传感和成像中广泛应用光学成像技术。这项工作全面概述了单个 PNP 的光学特性，描述了五种常用的光学成像技术，包括表面等离子体共振（SPR）显微镜、表面增强拉曼散射（SERS）技术、微分干涉对比（DIC）显微镜、全内反射散射（TIRS）显微镜和暗场显微镜（DFM）技术，重点介绍了它们在生物成像和传感中的单等离子体纳米探针及其应用机制，以及这些领域的挑战和未来趋势。
