Madec A, Javre J L, Haras D, Samperez S, Jouan P
Centre régional de recherche en endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
Bull Cancer. 1988;75(2):187-94.
Two isoenzymes of Thymidine Kinase were isolated from 90 human breast cancers by ion exchange chromatography. One was the enzyme present in adult tissues (TK-A), the second corresponded with the enzyme found in fetal tissues (TK-F). The presence of both isoenzymes was observed in the 90 tumors investigated. The activity of TK-A varied in the same range as in normal tissues, while the activity of TK-F varied to a larger extent and was higher than that of TK-A in a majority of tumors. Moreover, TK-F activity was independent of the presence or of the level of estradiol and progesterone receptors. Both isoenzymes were further characterized and compared.
通过离子交换色谱法从90例人类乳腺癌中分离出两种胸苷激酶同工酶。一种是存在于成人组织中的酶（TK-A），另一种与胎儿组织中发现的酶相对应（TK-F）。在所研究的90个肿瘤中均观察到两种同工酶的存在。TK-A的活性变化范围与正常组织相同，而TK-F的活性变化幅度更大，且在大多数肿瘤中高于TK-A。此外，TK-F的活性与雌二醇和孕酮受体的存在或水平无关。对这两种同工酶进行了进一步的表征和比较。
