Department of Gastroenterology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
Department of Pulmonary, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
J Int Med Res. 2020 Apr;48(4):300060519865368. doi: 10.1177/0300060519865368. Epub 2019 Dec 19.
To observe the effects of glutaminase (GLS) inhibitors on autophagy and proliferation of pancreatic stellate cells, and to explore their functions in pancreatic cancer.
Pancreatic cancer cells were divided into two groups. Group A was the control untreated group, and group B cells were treated with GLS inhibitors. Western blotting was used to detect the expression of Atg5, Bcl-2, Bax, and Bid proteins. The bromodeoxyuridine assay and scratch test were employed to investigate cell proliferation and invasion, respectively. The expression of E-cadherin, vimentin, cell adhesion molecule 2 (CADM2), and Snail protein was investigated by immunofluorescence.
The expression of Atg5, Bax, and Bid was higher in group A than in group B, while Bcl-2 expression was lower in group A than in group B. Group A cells demonstrated greater proliferation and invasion than group B cells. The expression of E-cadherin was lower in group A cells than group B cells, while vimentin, CADM2, and Snail were expressed at higher levels in group A than group B cells.
The inhibition of glutamine isozymes reduces autophagy and apoptosis in astrocytes, and inhibits pancreatic cancer cell proliferation and metastasis, while reducing their invasiveness.
观察谷氨酰胺酶（GLS）抑制剂对胰腺星状细胞自噬和增殖的影响，并探讨其在胰腺癌中的作用。
将胰腺癌细胞分为两组。A 组为对照组，未进行处理；B 组细胞用 GLS 抑制剂处理。采用 Western blot 法检测 Atg5、Bcl-2、Bax 和 Bid 蛋白的表达。采用溴脱氧尿苷检测法和划痕实验分别检测细胞增殖和侵袭情况。免疫荧光法检测 E-钙黏蛋白、波形蛋白、细胞黏附分子 2（CADM2）和 Snail 蛋白的表达。
与 B 组相比，A 组 Atg5、Bax 和 Bid 的表达更高，而 Bcl-2 的表达更低。A 组细胞的增殖和侵袭能力均强于 B 组。与 B 组相比，A 组细胞中 E-钙黏蛋白的表达更低，而波形蛋白、CADM2 和 Snail 的表达更高。
抑制谷氨酰胺同工酶可减少星形胶质细胞中的自噬和凋亡，抑制胰腺癌细胞的增殖和转移，同时降低其侵袭性。
