Department of Pharmacology, Johannes Gutenberg University Medical Center, Mainz, Germany.
Sci Rep. 2019 Dec 20;9(1):19556. doi: 10.1038/s41598-019-55987-x.
The aim of the present study was to compare different diets used to induce obesity in a head-to-head manner with a focus on insulin resistance and vascular dysfunction. Male C57BL/6J mice were put on standard chow diet (SCD), normal-fat diet (NFD), cafeteria diet (CAF) or high-fat diet (HFD) for 12 weeks starting at the age of 6 weeks. Both CAF and HFD led to obesity (weight gain of 179% and 194%, respectively), glucose intolerance and insulin resistance to a comparable extent. In aortas containing perivascular adipose tissue (PVAT), acetylcholine-induced vasodilation was best in the NFD group and worst in the CAF group. Reduced phosphorylation of endothelial nitric oxide synthase at serine 1177 was observed in both CAF and HFD groups. Plasma coagulation activity was highest in the HFD group and lowest in the SCD group. Even the NFD group had significantly higher coagulation activity than the SCD group. In conclusions, CAF and HFD are both reliable mouse diets in inducing visceral obesity, glucose intolerance and insulin resistance. CAF is more effective than HFD in causing PVAT dysfunction and vascular dysfunction, whereas hypercoagulability was mostly evident in the HFD group. Coagulation activity was higher in NFD than NCD group.
本研究旨在对头对头比较不同的饮食诱导肥胖方法，重点关注胰岛素抵抗和血管功能障碍。雄性 C57BL/6J 小鼠从 6 周龄开始分别给予标准饮食（SCD）、正常脂肪饮食（NFD）、自助餐饮食（CAF）或高脂肪饮食（HFD）12 周。CAF 和 HFD 均导致肥胖（体重分别增加 179%和 194%）、葡萄糖不耐受和胰岛素抵抗的程度相当。在含有血管周围脂肪组织（PVAT）的主动脉中，NFD 组乙酰胆碱诱导的血管舒张最好，CAF 组最差。CAF 和 HFD 组均观察到内皮型一氧化氮合酶丝氨酸 1177 的磷酸化减少。HFD 组的血浆凝血活性最高，SCD 组最低。甚至 NFD 组的凝血活性也明显高于 SCD 组。结论：CAF 和 HFD 都是诱导内脏肥胖、葡萄糖不耐受和胰岛素抵抗的可靠的小鼠饮食。CAF 比 HFD 更有效地引起 PVAT 功能障碍和血管功能障碍，而高凝状态主要在 HFD 组中明显。NFD 组的凝血活性高于 NCD 组。
