Department of Radiation Oncology, the Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen 518020, Guangdong, China.
Department of oncology, the first affiliated hospital of Ji Nan University, Guangzhou 510632, Guangdong, China.
Ann Diagn Pathol. 2020 Feb;44:151436. doi: 10.1016/j.anndiagpath.2019.151436. Epub 2019 Dec 11.
Despite development in therapies, the high recurrence and low positivity of biomarkers for diagnosis still result in glioma with high mortality. In this study, we aimed to identify a potential miRNA signature to evaluate the effect of radiotherapy in glioma patients. MicroRNA (miRNA) sequencing was performed on miRNAs isolated from serum exosomes in a cohort of glioma patients before and after radiotherapy. A total of 18 up-regulated differentially expressed (DE) miRNAs and 16 down-regulated DE miRNAs were identified. Subsequently, the target genes of DE miRNAs were predicted based on multiple miRNA-target databases. Further, it was indicated that these targets were primarily involved in metabolic process, p53 signaling pathway and cancer pathways, suggesting that these miRNAs play a crucial role in glioma by regulating targets and affect the occurrence and development of the disease. In general, this study presented the variation of miRNAs in blood exosomes before and after radiotherapy. It can not only be helpful for the diagnosis of glioma, but also find new candidate biomarkers for monitoring the condition and evaluating the efficacy of radiotherapy in glioma. It provides a new idea for the diagnosis, treatment and prognosis evaluation of glioma.
尽管治疗方法有所发展，但用于诊断的生物标志物的高复发率和低阳性率仍导致胶质瘤死亡率居高不下。在这项研究中，我们旨在确定一种潜在的 miRNA 特征，以评估放疗对胶质瘤患者的疗效。对放疗前后胶质瘤患者血清外泌体中分离的 miRNA 进行 miRNA 测序。共鉴定出 18 个上调差异表达（DE）miRNA 和 16 个下调 DE miRNA。随后，基于多个 miRNA 靶标数据库预测 DE miRNA 的靶基因。进一步表明，这些靶基因主要参与代谢过程、p53 信号通路和癌症通路，提示这些 miRNA 通过调节靶基因在胶质瘤中发挥重要作用，并影响疾病的发生和发展。总的来说，本研究展示了放疗前后血液外泌体中 miRNA 的变化。它不仅有助于胶质瘤的诊断，而且还可以找到新的候选生物标志物，用于监测病情和评估放疗对胶质瘤的疗效。为胶质瘤的诊断、治疗和预后评估提供了新思路。
