通过离心淘析对酿酒酵母细胞群体进行分级分离。
Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation.
作者信息
Gordon C N, Elliott S C
出版信息
J Bacteriol. 1977 Jan;129(1):97-100. doi: 10.1128/jb.129.1.97-100.1977.
Abstract
An exponential population of Saccharomyces cerevisiae cells was fractionated by centrifugal elutriation, using water as the elutriating liquid. Evidence that the population had been fractionated according to age in the cell cycle was obtained by examining the fractions for their size distribution, their microscopic appearance after Giemsa staining, and their ability to initiate synchronous growth.
摘要
使用水作为淘析液，通过离心淘析对指数生长的酿酒酵母细胞群体进行分级分离。通过检查各分级分离物的大小分布、吉姆萨染色后的显微镜外观以及它们启动同步生长的能力，获得了该群体已根据细胞周期中的年龄进行分级分离的证据。
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