Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, USA.
Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, USA.
Sci Rep. 2019 Dec 27;9(1):20001. doi: 10.1038/s41598-019-56454-3.
Premature ovarian insufficiency (POI) affects approximately 1% of women. We aim to understand the ovarian microenvironment, including the extracellular matrix (ECM) and associated proteins (matrisome), and its role in controlling folliculogenesis. We mapped the composition of the matrisome of porcine ovaries through the cortical compartment, where quiescent follicles reside and the medullary compartment, where the larger follicles grow and mature. To do this we sliced the ovaries, uniformly in two anatomical planes, enriched for matrisome proteins and performed bottom-up shotgun proteomic analyses. We identified 42 matrisome proteins that were significantly differentially expressed across depths, and 11 matrisome proteins that have not been identified in previous ovarian protein analyses. We validated these data for nine proteins and confirmed compartmental differences with a second processing method. Here we describe a processing and proteomic analysis pipeline that revealed spatial differences and matrisome protein candidates that may influence folliculogenesis.
卵巢早衰（POI）影响大约 1%的女性。我们旨在了解卵巢微环境，包括细胞外基质（ECM）和相关蛋白（matrisome）及其在控制卵泡发生中的作用。我们通过皮质隔室（静息卵泡所在的位置）和髓质隔室（较大卵泡生长和成熟的位置）描绘了猪卵巢的 matrisome 组成。为此，我们将卵巢切成均匀的两个解剖平面切片，富集 matrisome 蛋白，并进行自下而上的鸟枪法蛋白质组学分析。我们鉴定了 42 种在深度上差异表达的 matrisome 蛋白，和 11 种以前在卵巢蛋白质分析中未鉴定的 matrisome 蛋白。我们对 9 种蛋白质进行了验证，并使用第二种处理方法证实了隔室差异。在这里，我们描述了一个处理和蛋白质组学分析管道，该管道揭示了空间差异和可能影响卵泡发生的 matrisome 蛋白候选物。
