Department of Pathology, Huizhou Third People's Hospital, Guangzhou Medical University, Huizhou, Guangdong, China (mainland).
Department of Pathology, Yuebei People's Hospital, Shaoguan, Guangdong, China (mainland).
Med Sci Monit. 2019 Dec 28;25:10089-10094. doi: 10.12659/MSM.918288.
BACKGROUND Nectin-4 is overexpressed in several human malignant tumors. This study aimed to investigate the expression of Nectin-4 in esophageal cancer tissues compared with adjacent normal esophageal tissue and its association with clinicopathological parameters and prognosis. MATERIAL AND METHODS Nectin-4 expression in esophageal cancer tissues was compared with adjacent normal esophageal tissue from 94 patients using immunohistochemistry, Western blot, and quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The chi-squared (χ²) test and Fisher's exact test compared categorical variables. The log-rank test and Kaplan-Meier survival analysis assessed the relationship between Nectin-4 expression and overall survival (OS). Univariate and multivariate Cox proportional risk models compared Nectin-4 expression, patient prognosis, and clinicopathological parameters. RESULTS Nectin-4 expression was significantly increased in esophageal cancer tissue compared with normal tissue (P<0.001), tumor size ≥4.5 cm, and tumor invasion in T3/T4 compared with T1/T2 stage. Kaplan-Meier survival analysis showed that the OS of patients with increased Nectin-4 expression was significantly reduced compared with patients with low levels of Nectin-4 expression. Patient prognosis in men was less than women, tumor diameter ≥4.5 cm, lymph node involvement, and depth of invasion were associated with poor prognosis. Independent prognostic factors were Nectin-4 expression, lymph node involvement, and depth of invasion. CONCLUSIONS In patients with esophageal cancer, the expression levels of Nectin-4, lymph node involvement, and depth of tumor invasion were independent prognostic factors. Further studies should be performed to evaluate the diagnostic and prognostic roles of Nectin-4 and its potential role as a therapeutic target.
Nectin-4 在多种人类恶性肿瘤中过表达。本研究旨在探讨 Nectin-4 在食管癌组织中的表达情况，并与相邻正常食管组织进行比较，分析其与临床病理参数及预后的关系。
采用免疫组织化学、Western blot 和实时定量逆转录聚合酶链反应（RT-qPCR）检测 94 例食管癌组织及相邻正常食管组织中 Nectin-4 的表达情况，比较 Nectin-4 在食管癌组织中的表达与正常组织的差异，分析其与临床病理参数及预后的关系。采用卡方检验（χ² 检验）和 Fisher 确切概率法比较分类变量，采用 log-rank 检验和 Kaplan-Meier 生存分析评估 Nectin-4 表达与总生存（OS）的关系，采用单因素和多因素 Cox 比例风险模型比较 Nectin-4 表达、患者预后及临床病理参数。
Nectin-4 在食管癌组织中的表达明显高于正常组织（P<0.001），肿瘤大小≥4.5cm 和 T3/T4 期肿瘤浸润与 T1/T2 期相比明显增加。Kaplan-Meier 生存分析显示，Nectin-4 高表达患者的 OS 明显低于 Nectin-4 低表达患者。男性患者的预后不如女性患者，肿瘤直径≥4.5cm、淋巴结受累和浸润深度与不良预后相关。独立的预后因素是 Nectin-4 表达、淋巴结受累和浸润深度。
在食管癌患者中，Nectin-4 的表达水平、淋巴结受累和肿瘤浸润深度是独立的预后因素。进一步的研究应评估 Nectin-4 的诊断和预后作用及其作为治疗靶点的潜在作用。
