Fokam Tagne Michel Archange, Akaou Hypolyte, Noubissi Paul Aimé, Foyet Fondjo Angèle, Rékabi Yaouke, Wambe Henri, Kamgang René, Essame Oyono Jean-Louis
Department of Biological Science, Faculty of Science, University of Ngaoundere, Cameroon.
Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Cameroon.
Gastroenterol Res Pract. 2019 Dec 1;2019:6963548. doi: 10.1155/2019/6963548. eCollection 2019.
The treatment of diarrheal diseases is a serious problem in developing countries, where population generally uses medicinal plants. The leaves of have been reported to be traditionally used in the treatment of diarrhea by local people in the district of Khulna in Bangladesh. The aim of this study was to investigate the effects of the hydroethanolic extract of leaves on castor oil-induced diarrhea in mice.
The powder of the leaves of was macerated in ethanol/water mixture (20/80) for 48 hours and then filtered. The filtrate obtained was lyophilized, and the solutions to be administered to the animals were prepared. To induce diarrhea, animals orally received castor oil (1 mL/100 g bw). To determine the effective doses, each mouse received, 30 minutes after the administration of castor oil, one of the single oral doses of hydroethanolic extract of leaves: 0, 25, 50, 100, and 200 mg/kg bw. The mass, number, and frequency of stool diarrhea were measured and recorded per hour for five hours. The effect of the hydroethanolic extract of leaves on the intestinal transit was evaluated by measuring the distance traveled by the charcoal meal in thirty minutes. The effects of the aqueous extract of hydroethanolic extract of leaves on intestinal secretion were evaluated by measuring the volume of the intestinal content and by dosing the electrolytes (Na, K, and Cl) in the intestinal content by the colorimetric method.
The extract produced significant ( < 0.01) decreases, respectively, 35.52%, 54.47%, 74.80%, and 87.80% in the severity of diarrhea. The extract at 100 and 200 mg/kg bw showed a significant ( < 0.01) decrease of castor oil-induced enteropooling (61.08% and 65.41%), and only the 200 mg/kg bw exhibited significant ( < 0.01) reduction on intestinal transit (24.46%) as compared to standard drug.
The hydroethanolic extract was found to be effective against castor oil-induced diarrhea in experimental mice at 50, 100, and 200 mg/kg bw which provides evidence that could justify its traditional use.
在发展中国家，腹泻疾病的治疗是一个严重问题，这些国家的民众普遍使用药用植物。据报道，孟加拉国库尔纳地区的当地人传统上使用[植物名称]的叶子治疗腹泻。本研究旨在调查[植物名称]叶子的水乙醇提取物对蓖麻油诱导的小鼠腹泻的影响。
将[植物名称]的叶子粉末在乙醇/水混合物（20/80）中浸泡48小时，然后过滤。将得到的滤液冻干，制备给动物给药的溶液。为诱导腹泻，动物口服蓖麻油（1 mL/100 g体重）。为确定有效剂量，每只小鼠在给予蓖麻油30分钟后，口服单剂量的[植物名称]叶子水乙醇提取物：0、25、50、100和200 mg/kg体重。每小时测量并记录5小时内粪便腹泻的质量、数量和频率。通过测量炭末在30分钟内移动的距离来评估[植物名称]叶子水乙醇提取物对肠道转运的影响。通过测量肠内容物体积并采用比色法测定肠内容物中的电解质（钠、钾和氯）来评估[植物名称]叶子水乙醇提取物的水提取物对肠道分泌的影响。
提取物分别使腹泻严重程度显著降低（P<0.01），降低幅度为35.52%、54.47%、74.80%和87.80%。100和200 mg/kg体重的提取物使蓖麻油诱导的肠积液显著降低（P<0.01）（分别为61.08%和65.41%），与标准药物相比，只有200 mg/kg体重的提取物使肠道转运显著降低（P<0.01）（24.46%）。
发现水乙醇提取物在50、100和200 mg/kg体重时对蓖麻油诱导的实验小鼠腹泻有效，这为其传统用途提供了合理依据。
