Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, 510006, Guangdong Province, China.
Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, 510006, Guangdong Province, China; Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, China.
Carbohydr Polym. 2020 Feb 15;230:115640. doi: 10.1016/j.carbpol.2019.115640. Epub 2019 Nov 19.
Bone transplantations are still facing many serious challenges, hydrogel as a new kind of artificial bone substitutes has developed into a promising bone scaffold material. However, it is still a challenge to combine bioactive agents and hydrogel matrix to promote osteoinductivity. Herein, we developed a novel bioactive hydrogel based on arginine-based unsaturated poly (ester amide) (Arg-UPEA) and methacrylated hyaluronic acid (HA-MA) via photo-crosslinking. As the results indicated, we found that the introduction of Arg-UPEA into HA-MA hydrogels could finely modulate their compressive modulus, swelling level and porous structure. Besides, among groups of different feed ratio, groups of 10 % and 15 % of Arg-UPEA content effectively enhanced osteogenic differentiation in osteoblasts when compared with HA-MA hydrogel. Furthermore, better bone regeneration and expression of osteogenesis-related factors in vivo also verified the Arg-UPEA/HA-MA hybrid hydrogels as a promising scaffold material for bone tissue engineering.
骨移植仍然面临许多严峻的挑战，水凝胶作为一种新型的人工骨替代品，已发展成为一种很有前途的骨支架材料。然而，将生物活性剂与水凝胶基质结合以促进成骨诱导仍然是一个挑战。在此，我们通过光交联法开发了一种新型的基于精氨酸的不饱和聚酯酰胺（Arg-UPEA）和甲基丙烯酰化透明质酸（HA-MA）的新型生物活性水凝胶。结果表明，我们发现将 Arg-UPEA 引入 HA-MA 水凝胶中可以精细调节其压缩模量、溶胀水平和多孔结构。此外，在不同的进料比组中，与 HA-MA 水凝胶相比，Arg-UPEA 含量为 10%和 15%的组能有效增强成骨细胞的成骨分化。此外，体内更好的骨再生和骨形成相关因子的表达也验证了 Arg-UPEA/HA-MA 杂化水凝胶作为骨组织工程有前途的支架材料。
