Laboratório de Sanidade Animal, Hospital Veterinário, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Laboratório de Fisiologia e Bioquímica de Microrganismos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil.
J Microbiol Biotechnol. 2020 Apr 28;30(4):540-551. doi: 10.4014/jmb.1907.07064.
Sporotrichosis is a chronic and subacute mycosis causing epidemiological outbreaks involving sick cats and humans in southeastern Brazil. The systemic disease prevails in cats, and in humans, the symptoms are restricted to skin in immunocompetent individuals. Under these conditions, the prolonged treatment of animals and cases of recurrence justify the discovery of new treatments for sporotrichosis. This work addresses the antifungal activity of silver salts of Keggin-type heteropolyacid salts (Ag-HPA salts) such as Ag[PWO], Ag[SiWVO], Ag[SiWO] and Ag[PMoO] and interactions with the antifungal drugs itraconazole (ITC), terbinafine (TBF) and amphotericin B (AMB) on the yeast and mycelia forms of spp. spp. yeast cells were susceptible to Ag-HPA salts at minimum inhibitory concentration (MIC) values ranging from 8 to 128 μg/mL. Interactions between Ag[PWO] and Ag[PMoO] with itraconazole and amphotericin B resulted in higher antifungal activity with a reduction in growth and melanization. Treated cells showed changes in cell membrane integrity, vacuolization, cytoplasm disorder, and membrane detachment. Promising antifungal activity for treating sporotrichosis was observed for the Ag-HPA salts Ag[PMoO] and Ag[PWO], which have a low cost, high yield and activity at low concentrations. However, further evaluation of in vivo tests is still required.
孢子丝菌病是一种慢性和亚急性真菌病，在巴西东南部会引起涉及患病猫和人类的流行病学暴发。系统性疾病在猫中流行，而在免疫功能正常的个体中，症状仅限于皮肤。在这些情况下，动物的长期治疗和复发病例证明了需要发现新的孢子丝菌病治疗方法。这项工作涉及 Keggin 型杂多酸银盐（Ag-HPA 盐）的抗真菌活性，例如 Ag[PWO]、Ag[SiWVO]、Ag[SiWO]和 Ag[PMoO]，以及与抗真菌药物酮康唑（ITC）、特比萘芬（TBF）和两性霉素 B（AMB）在酵母和菌丝形式的 中的相互作用。 酵母细胞对 Ag-HPA 盐的最低抑菌浓度（MIC）值在 8 至 128 μg/mL 范围内敏感。Ag[PWO]和 Ag[PMoO]与酮康唑和两性霉素 B 的相互作用导致更高的抗真菌活性，同时降低了生长和黑色素化。处理后的细胞显示出细胞膜完整性、空泡化、细胞质紊乱和膜脱落的变化。Ag-HPA 盐 Ag[PMoO]和 Ag[PWO]表现出有前途的抗孢子丝菌病活性，它们具有低成本、高产量和低浓度下的活性。然而，仍需要进一步评估体内试验。
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