De Almeida Adriana Jardim, Nahn Júnior Edilbert Pelegrini, Vieira da Motta Olney, Lourenço Camila da Silva, Bernardino Maria de Lourdes Amaral, Nahn Gabriel Portal Barros Pellegrini
Center for Agricultural Science and Technology, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil.
Adult Health Department, Campos School of Medicine, Campos dos Goytacazes, RJ, Brazil.
J Infect Dev Ctries. 2019 Aug 31;13(8):768-772. doi: 10.3855/jidc.11672.
Sporotrichosis is an infectious fungal zoonosis associated with traumatic implantation in the skin of dimorphic fungi of the Sporothrix schenckii complex. The objective of this study was to diagnose sporotrichosis in patients in the city of Campos dos Goytacazes, and to establish correlations between positive laboratory results and dermatological and topographical aspects of the lesions and personal traits of the patients, such as sex, age and profession.
We collected samples from 22 patients with suspect lesions, which were sent to the laboratory for direct microscopic analysis after Gram staining, followed by mycological culture by seeding the material in 4% Sabouraud dextrose agar (Himedia®) supplemented with chloramphenicol (50 mg/Lt) and cycloheximide (400 mg/Lt - Sigma-Aldrich, USA). The dishes were incubated at 25-30oC. For confirmation of the diagnosis, the strains isolated in mycelial form were converted to yeast form by culture in yeast-peptone-dextrose (YPD) agar at 37oC for up to 15 days.
The positive results demonstrated that the disease was most frequently diagnosed in women between the ages of 19 and 60 years, and that 18 (81.8%) of the patients reported having contact with pet cats. The occupations of those positive for sporotrichosis were not related to the disease. The upper limbs were the body region most often afflicted, with observation in the majority of cases of ulcerated lesions, although five patients also had nodular lesions. Additionally, the observation of lymphatic cords was frequent.
In recent years, sporotrichosis has been diagnosed with relative frequency in Campos dos Goytacazes, causing great concern among public health officials and practitioners.
孢子丝菌病是一种传染性真菌人畜共患病，与申克孢子丝菌复合体的双相真菌经创伤植入皮肤有关。本研究的目的是诊断戈亚卡泽斯营地市患者的孢子丝菌病，并建立实验室阳性结果与病变的皮肤学和地形学特征以及患者的个人特征（如性别、年龄和职业）之间的相关性。
我们从22例有可疑病变的患者中采集样本，样本经革兰氏染色后送实验室进行直接显微镜分析，然后将材料接种于添加氯霉素（50mg/L）和环己酰亚胺（400mg/L - 美国西格玛奥德里奇公司）的4%沙氏葡萄糖琼脂（希美迪®）中进行真菌培养。培养皿在25 - 30℃下孵育。为确诊，将以菌丝形式分离的菌株在37℃的酵母蛋白胨葡萄糖（YPD）琼脂中培养长达15天，使其转化为酵母形式。
阳性结果表明，该病最常诊断于19至60岁的女性，18例（81.8%）患者报告与宠物猫有接触。孢子丝菌病阳性患者的职业与该病无关。上肢是最常受累的身体部位，大多数病例观察到溃疡性病变，不过有5例患者也有结节性病变。此外，经常观察到淋巴管索。
近年来，戈亚卡泽斯营地市相对频繁地诊断出孢子丝菌病，引起了公共卫生官员和从业者的极大关注。
