Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Prêto, São Paulo, Brazil.
Division of Plastic Surgery of Ribeirão Preto Medical School, Department of Surgery and Anatomy, University of São Paulo, Ribeirão Preto, Brazil.
Aesthetic Plast Surg. 2020 Jun;44(3):971-978. doi: 10.1007/s00266-019-01586-0. Epub 2020 Jan 2.
New regenerative treatments have emerged with the use of multipotent mesenchymal cells, with special interest in adipose-derived stem cells (ADSCs). In recent years, studies that have sought to identify possible quantitative or qualitative differences in ADSCs derived from different donor subcutaneous adipose tissue have shown divergent results making the determination of a preferential donor area still considered inconclusive.
The number of ADSCs present in the adipose tissue collected by liposuction was quantified between five different body areas from 17 women, by means of the CFU-F assay and to investigate possible qualitative differences in the ADSCs from these different areas by analyzing: cell surface markers, cell kinetics, action of the supernatant produced by ADSCs from different body areas on fibroblast migration and, finally, differences in the secretome present in the supernatant produced by these cells.
The highest mean concentration of CFU-Fs was the dorsum (23.20 ± 26.13), and the lowest was the thighs (6.87 ± 5.04). No qualitative differences were observed in the expression of the cell surface markers or in cell kinetics. Supernatants produced by the ADSCs derived from the abdomen and the thighs demonstrated an increased rate of migration of fibroblasts in vitro similarly. No differences were observed in the secretome between the ADSCs groups.
It was observed that the region of the dorsal upper back presented a greater number of ADSCs than the thighs. No qualitative differences were observed between the ADSCs of the five areas analyzed.
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随着多能间充质细胞的使用，新的再生治疗方法已经出现，特别关注脂肪来源的干细胞（ADSCs）。近年来，一些研究试图确定源自不同供体皮下脂肪的 ADSC 中可能存在的定量或定性差异，但结果却存在分歧，这使得确定首选供体区域的问题仍然没有定论。
通过 CFU-F 测定，从 17 名女性的五个不同身体区域的脂肪组织中定量检测 ADSC 的数量，并通过分析以下方面来研究这些不同区域的 ADSC 可能存在的定性差异：细胞表面标志物、细胞动力学、来自不同身体区域的 ADSC 产生的上清液对成纤维细胞迁移的作用，以及最后，分析这些细胞产生的上清液中存在的不同的分泌组。
CFU-F 最高的平均浓度位于背部（23.20±26.13），最低的位于大腿（6.87±5.04）。细胞表面标志物的表达或细胞动力学方面未观察到定性差异。来自腹部和大腿的 ADSC 产生的上清液在体外显示出类似的成纤维细胞迁移率增加。ADSC 组之间的分泌组没有差异。
观察到背部上背部区域的 ADSC 数量多于大腿。在分析的五个区域的 ADSC 之间没有观察到定性差异。
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