The Type VI Secretion System Enhances the Oxidative Stress Response and Host Colonization.

The role of the Type VI secretion system (T6SS) in is poorly understood despite an increasing prevalence of the T6SS in recent isolates in humans and chickens. The T6SS is a contractile secretion machinery capable of delivering effectors that can play a role in host colonization and niche establishment. During host colonization, is exposed to oxidative stress in the host gastrointestinal tract, and in other bacteria the T6SS has been linked with the oxidative stress response. In this study, comparisons of whole genome sequences of a novel human isolate 488 with previously sequenced strains revealed a single highly conserved T6SS cluster shared between strains isolated from humans and chickens. The presence of a functional T6SS in the 488 wild-type strain is indicated by expression of T6SS genes and secretion of the effector TssD. Increased expression of oxidative stress response genes , , and , and increased oxidative stress resistance in 488 wild-type strain suggest T6SS is associated with oxidative stress response. The role of the T6SS in interactions with host cells is explored using and models, and the presence of the T6SS is shown to increase cytotoxicity in the infection model. In biologically relevant models, the T6SS enhances interactions with and invasion of chicken primary intestinal cells and enhances the ability of to colonize chickens. This study demonstrates that the T6SS provides defense against oxidative stress and enhances host colonization, and highlights the importance of the T6SS during survival of T6SS-positive strains.