Fehlbaum Sophie, Chassard Christophe, Schwab Clarissa, Voolaid Maarja, Fourmestraux Candice, Derrien Muriel, Lacroix Christophe
Laboratory of Food Biotechnology, Institute of Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland.
Danone Nutricia Research, Palaiseau, France.
Front Nutr. 2019 Dec 10;6:184. doi: 10.3389/fnut.2019.00184. eCollection 2019.
Consumption of probiotic bacteria can result in a transient colonization of the human gut and thereby in potential interactions with the commensal microbiota. In this study, we used novel PolyFermS continuous fermentation models to investigate interactions of the candidate probiotic strain CNCM I-1518 () with colonic microbiota from healthy elderly subjects using 16S rRNA gene amplicon sequencing and metatranscriptomics, or with microbiota -colonized with ( NCTC 13307 and DSM 1296)-an enteropathogen prevalent in the elderly population. Small changes in microbiota composition were detected upon daily addition of , including increased abundances of closely related genera and , and of the butyrate producer . Microbiota gene expression was also modulated by with distinct response of the transcriptome and an increase in carbohydrate utilization. However, no inhibitory effect of was observed on colonization in the intestinal models under the tested conditions. Our data suggest that, in the experimental conditions tested and independent of the host, has modulatory effects on both the composition and function of elderly gut microbiota without affecting growth and toxin production.
食用益生菌可导致人体肠道的短暂定殖，从而可能与共生微生物群发生相互作用。在本研究中，我们使用新型PolyFermS连续发酵模型，通过16S rRNA基因扩增子测序和宏转录组学，研究候选益生菌菌株CNCM I-1518（）与健康老年受试者的结肠微生物群之间的相互作用，或与由（NCTC 13307和DSM 1296）定殖的微生物群（一种在老年人群中普遍存在的肠道病原体）之间的相互作用。每天添加后，检测到微生物群组成有微小变化，包括密切相关属和的丰度增加，以及丁酸盐产生菌的丰度增加。微生物群基因表达也受到的调节，转录组有明显反应，碳水化合物利用率增加。然而，在测试条件下，未观察到对肠道模型中定殖的抑制作用。我们的数据表明，在测试的实验条件下且与宿主无关，对老年肠道微生物群的组成和功能具有调节作用，而不影响其生长和毒素产生。
