Department of Safety and Quality of Meat, Max Rubner-Institut, E.-C.-Baumann-Strasse 20, D-95326 Kulmbach, Germany.
Center for Biotechnology - CeBiTec, Bielefeld University, Universitätsstraße 27, D-33615 Bielefeld, Germany.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1528-1540. doi: 10.1099/ijsem.0.003928.
During investigations of spoilage-associated meat microbiota, isolates were found in two different laboratories showing highest similarities to DSM 29167, DSM 29164 and DSM 18862 based on 16S rRNA gene sequence comparisons. Phylogenetic analysis of the complete gene sequences of isolates B4-1 and SpeckC indicated a separate branch with 99.0 and 99.1 % identity, respectively, to their closest relative ( DSM 29167). Further phenotypic and chemotaxonomic characterizations, as well as average nucleotide identity (ANIb) values obtained from the draft genomes, revealed that these isolates could be considered as representing a novel species, with ANIb values of around 94 and 90 % with their closest relatives and . Other related species showed ANIb values below 90 %, including DSM 17149, DSM 18928, DSM 17489, DSM 11331 and DSM 18862. Genome-to-genome distance calculations between B4-1 and its closest relative, DSM 29167, showed 62.6 % relatedness. The G+C contents of B4-1 and SpeckC were 59.8 and 59.9 mol%, respectively. The major cellular lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q9. Based on these data, the new species sp. nov. is proposed, the type strain is B4-1 (=DSM 107652=LMG 30892); a second strain is SpeckC (=DSM 107651=LMG 30893).
在对变质相关的肉菌群进行调查时,两个不同的实验室发现了一些分离株,这些分离株与 DSM 29167、DSM 29164 和 DSM 18862 基于 16S rRNA 基因序列比较的相似度最高。分离株 B4-1 和 SpeckC 的全 基因序列的系统发育分析表明,它们与最接近的亲缘种(DSM 29167)的相似度分别为 99.0%和 99.1%,形成单独的分支。进一步的表型和化学分类特征,以及从草图基因组中获得的平均核苷酸同一性(ANIb)值表明,这些分离株可以被认为是代表一个新的物种,与最接近的亲缘种 DSM 29167 和 DSM 18862 的 ANIb 值约为 94%和 90%。其他相关物种的 ANIb 值低于 90%,包括 DSM 17149、DSM 18928、DSM 17489、DSM 11331 和 DSM 18862。B4-1 与其最接近的亲缘种 DSM 29167 之间的基因组到基因组距离计算显示,它们的亲缘关系为 62.6%。B4-1 和 SpeckC 的 G+C 含量分别为 59.8%和 59.9%。主要的细胞脂类是磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油;主要的醌是 Q9。基于这些数据,提出了新的物种 sp. nov.,其模式株是 B4-1(=DSM 107652=LMG 30892);第二株是 SpeckC(=DSM 107651=LMG 30893)。