Cailleau Guillaume, Hanson Buck T, Cravero Melissa, Zhioua Sami, Hilpish Patrick, Ruiz Celia, Robinson Aaron J, Kelliher Julia M, Morales Demosthenes, Gallegos-Graves La Verne, Bonito Gregory, Chain Patrick S G, Bindschedler Saskia, Junier Pilar
Laboratory of Microbiology, University of Neuchâtel, Neuchâtel, Switzerland.
Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States.
Front Fungal Biol. 2023 Dec 13;4:1285531. doi: 10.3389/ffunb.2023.1285531. eCollection 2023.
Members of the fungal genus are widely known for their important ecological roles and significant economic value. In this study, we used amplicon and genome sequencing to characterize bacterial communities associated with sexual fruiting bodies from wild specimens, as well as vegetative mycelium and sclerotia obtained from isolates grown . These investigations included diverse representatives from both Elata and Esculenta clades. Unique bacterial community compositions were observed across the various structures examined, both within and across individual isolates or specimens. However, specific bacterial taxa were frequently detected in association with certain structures, providing support for an associated core bacterial community. Bacteria from the genus and constituted the core bacterial associates of mycelia and sclerotia, while other genera (e.g., spp., spp., and spp.) constituted the core bacterial community of fruiting bodies. Furthermore, the importance of as a key member of the bacteriome was supported by the isolation of several strains from mycelia during cultivation. Four of the six mycelial-derived isolates shared 16S rDNA sequence identity with amplicon sequences recovered directly from the examined fungal structures. Distinct interaction phenotypes (antagonistic or neutral) were observed in confrontation assays between these bacteria and various isolates. Genome sequences obtained from these isolates revealed intriguing differences in gene content and annotated functions, specifically with respect to toxin-antitoxin systems, cell adhesion, chitinases, and insecticidal toxins. These genetic differences correlated with the interaction phenotypes. This study provides evidence that spp. are frequently associated with and these associations may greatly impact fungal physiology.
真菌属的成员因其重要的生态作用和显著的经济价值而广为人知。在本研究中，我们使用扩增子和基因组测序来表征与野生标本的有性子实体相关的细菌群落，以及从培养的分离物中获得的营养菌丝体和菌核。这些研究包括来自高卢松露（Tuber elata）和夏块菌（Tuber aestivum）进化枝的不同代表。在检查的各种结构中，无论是在单个分离物或标本内部还是之间，都观察到了独特的细菌群落组成。然而，特定的细菌分类群经常与某些结构相关联，这为相关的核心细菌群落提供了支持。伯克霍尔德氏菌属（Burkholderia）和泛菌属（Pantoea）的细菌构成了松露菌丝体和菌核的核心细菌伴生菌，而其他属（如假单胞菌属（Pseudomonas spp.）、芽孢杆菌属（Bacillus spp.）和链霉菌属（Streptomyces spp.））构成了子实体的核心细菌群落。此外，在培养过程中从菌丝体中分离出几株伯克霍尔德氏菌菌株，支持了伯克霍尔德氏菌作为细菌群落关键成员的重要性。六个菌丝体来源的伯克霍尔德氏菌分离物中有四个与直接从检查的真菌结构中回收的扩增子序列具有16S rDNA序列同一性。在这些细菌与各种松露分离物之间的对峙试验中观察到了不同的相互作用表型（拮抗或中性）。从这些伯克霍尔德氏菌分离物获得的基因组序列揭示了基因含量和注释功能方面的有趣差异，特别是在毒素-抗毒素系统、细胞粘附、几丁质酶和杀虫毒素方面。这些遗传差异与相互作用表型相关。本研究提供了证据，表明伯克霍尔德氏菌属物种经常与松露相关联，并且这些关联可能会极大地影响真菌生理学。
