School of Life Sciences, Northeast Normal University, Changchun, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3049-3054. doi: 10.1099/ijsem.0.004129. Epub 2020 Apr 3.
A short rod-shaped, Gram-stain-negative strain that can degrade multiple polymers was isolated from forest soil in China and designated as DSWY01. The results of 16S rRNA gene sequence analysis showed that this isolate shared high similarities with NBRC 102411 (99.3 %), NBRC 14162 (99.2%) and NBRC 13583 (99.0%). The results of phylogenetic analysis based on 16S rRNA gene sequence and multilocus sequence analysis (, , , and ) indicated that strain DSWY01 belongs to the genus and is a member of the group in an independent branch. The average nucleotide identity and digital DNA-DNA hybridization between the genome of strain DSWY01 and the genomes of other species (ANIb 77.72-89.65 %; GGDC 15.50-31.10 %) showed that the isolate represents a novel species. The DNA G+C content of strain DSWY01 was 63.67 mol%, and the major cellular fatty acids (>15 %) were a mixture of Cω7/Cω6 and C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified lipids, and the major quinone was CQ-10. The morphological, physiological and biochemical characteristics of the isolate were then compared with those of reference type strains. The isolate differed considerably from its closest relatives and is representative of a novel species of , for which the name sp. nov. is proposed. The type strain is DSWY01 (=DSM 106702=CCTCC AB 2018053).
一株能够降解多种聚合物的短杆状、革兰氏阴性菌株，从中国森林土壤中分离得到，并被命名为 DSWY01。16S rRNA 基因序列分析结果表明，该分离株与 NBRC 102411（99.3%）、NBRC 14162（99.2%）和 NBRC 13583（99.0%）具有高度相似性。基于 16S rRNA 基因序列和多位点序列分析（、、、和）的系统发育分析结果表明，菌株 DSWY01 属于 属，是 组中的一个独立分支的成员。菌株 DSWY01 与其他物种基因组之间的平均核苷酸同一性和数字 DNA-DNA 杂交值（ANIb 77.72-89.65%；GGDC 15.50-31.10%）表明该分离株代表了一个新的物种。菌株 DSWY01 的 DNA G+C 含量为 63.67mol%，主要细胞脂肪酸（>15%）为 Cω7/Cω6 和 C 的混合物。极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和两种未鉴定的脂类，主要醌为 CQ-10。然后将该分离株的形态学、生理学和生物化学特征与参考模式菌株进行比较。该分离株与最接近的亲缘关系菌株有很大的不同，代表了一个新的 属种，命名为 sp. nov.。模式菌株为 DSWY01（=DSM 106702=CCTCC AB 2018053）。
